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Literature summary for 2.1.1.204 extracted from

  • Tuorto, F.; Herbst, F.; Alerasool, N.; Bender, S.; Popp, O.; Federico, G.; Reitter, S.; Liebers, R.; Stoecklin, G.; Groene, H.J.; Dittmar, G.; Glimm, H.; Lyko, F.
    The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis (2015), EMBO J., 34, 2350-2362.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information generation of Dnmt2/- knockout mice Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs ?
-
?
additional information Mus musculus C57BL/6 Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs ?
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC Mus musculus Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC Mus musculus C57BL/6 Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC Mus musculus
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC Mus musculus C57BL/6
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC Mus musculus
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC Mus musculus C57BL/6
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus O55055
-
-
Mus musculus C57BL/6 O55055
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipocyte
-
Mus musculus
-
bone marrow cell primary Mus musculus
-
fibroblast
-
Mus musculus
-
haematopoietic cell
-
Mus musculus
-
hematopoietic progenitor cell Lin(neg) Sca1+cKit+ haematopoietic progenitor cells that represent a heterogeneous cell population containing the most primitive haematopoietic cells that have self-renewal capacity and haematopoietic progenitors that give rise to all mature cell types found in peripheral blood for a lifetime Mus musculus
-
mesenchymal stromal cell
-
Mus musculus
-
additional information enzyme Dnmt2 is expressed in various mouse tissues Mus musculus
-
osteoblast
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs Mus musculus ?
-
?
additional information Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs Mus musculus C57BL/6 ?
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
-
Mus musculus S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons Mus musculus S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
-
Mus musculus C57BL/6 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons Mus musculus C57BL/6 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
-
Mus musculus S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
-
Mus musculus C57BL/6 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
-
Mus musculus S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
-
Mus musculus C57BL/6 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
?

Synonyms

Synonyms Comment Organism
Dnmt2
-
Mus musculus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Mus musculus

General Information

General Information Comment Organism
malfunction specific codon mistranslation by tRNAs lacking Dnmt2-dependent methylation causes systematic differences in protein expression, with 153 significantly deregulated proteins among a total of 4094 identified proteins. Enzyme-deficient Dnmt2-/- mice reveal delayed endochondral ossification of the long bones with uantitative differences in the length of the zone of cell maturation and hypertrophy in the epiphyseal plate between wild-type and Dnmt2-/- mice as well as in the length of the trabecular zone between wild-type and Dnmt2-/- mice. The trabecular structures are not only reduced in length but also appear incorrectly interfaced with bone marrow cells. The numbers of capillaries counted in the trabecular zone are significantly reduced. Enzyme-deficient bone marrow cells show an enlarged pool of osteo-progenitors. Haematopoietic phenotype, detailed overview Mus musculus
physiological function Dnmt2 plays an important role in haematopoiesis and define an additional function of C38 tRNA methylation in the discrimination of near-cognate codons, thereby ensuring accurate polypeptide synthesis, role for Dnmt2 in the regulation of codon fidelity. The enzyme prevents tRNA fragmentation. Enzyme Dnmt2 is required for cell differentiation, e.g. of bone marrow mesenchymal stromal cells and for cell-autonomous differentiation during haematopoiesis Mus musculus