Protein Variants | Comment | Organism |
---|---|---|
D49A | abolishes almost completely the formation of 2'-O-methylcytidine32, no activity is detected for guanine34 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA | Saccharomyces cerevisiae | the enzyme catalyses the formation of 2'-O-methylnucleotides at positions 32 and 34 of the yeast tRNAPhe, tRNATrp and, possibly, tRNALeu | S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P38238 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant strain D49A | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA | the enzyme catalyses the formation of 2'-O-methylnucleotides at positions 32 and 34 of the yeast tRNAPhe, tRNATrp and, possibly, tRNALeu | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA | - |
? | |
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA | Trm7p methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, both in vivo and in vitro | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trm7p | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | a trm7DELTA strain, which is viable but grows slowly, translation is impaired. Protein synthesis is reduced to 30% in the deleted strain, compared with an isogenic wild-type strain. The formation of 2'-O-methylcytidine32 and guanosine34 is abolished in the trm7DELTA strain | Saccharomyces cerevisiae |