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Literature summary for 2.1.1.205 extracted from

  • Pintard, L.; Lecointe, F.; Bujnicki, J.M.; Bonnerot, C.; Grosjean, H.; Lapeyre, B.
    Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop (2002), EMBO J., 21, 1811-1820.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D49A abolishes almost completely the formation of 2'-O-methylcytidine32, no activity is detected for guanine34 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Saccharomyces cerevisiae 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA Saccharomyces cerevisiae the enzyme catalyses the formation of 2'-O-methylnucleotides at positions 32 and 34 of the yeast tRNAPhe, tRNATrp and, possibly, tRNALeu S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA
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?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P38238
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant strain D49A Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA the enzyme catalyses the formation of 2'-O-methylnucleotides at positions 32 and 34 of the yeast tRNAPhe, tRNATrp and, possibly, tRNALeu Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA
-
?
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA Trm7p methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, both in vivo and in vitro Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA
-
?

Synonyms

Synonyms Comment Organism
Trm7p
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction a trm7DELTA strain, which is viable but grows slowly, translation is impaired. Protein synthesis is reduced to 30% in the deleted strain, compared with an isogenic wild-type strain. The formation of 2'-O-methylcytidine32 and guanosine34 is abolished in the trm7DELTA strain Saccharomyces cerevisiae