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Literature summary for 2.1.1.217 extracted from

  • Roovers, M.; Kaminska, K.H.; Tkaczuk, K.L.; Gigot, D.; Droogmans, L.; Bujnicki, J.M.
    The YqfN protein of Bacillus subtilis is the tRNA: m1A22 methyltransferase (TrmK) (2008), Nucleic Acids Res., 36, 3252-3262.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.217

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine22 in tRNA Bacillus subtilis a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P54471
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine22 in Bacillus subtilis tRNA(Ser) crude Escherichia coli tRNA is used, since it does not contain m1A22 Bacillus subtilis S-adenosyl-L-homocysteine + N1-methyladenine22 in Bacillus subtilis tRNA(Ser)
-
 ?
S-adenosyl-L-methionine + adenine22 in tRNA a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules Bacillus subtilis S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
 ?
S-adenosyl-L-methionine + adenine22 in tRNA crude Escherichia coli tRNA is used, since it does not contain m1A22 Bacillus subtilis S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
 ?

Synonyms

Synonyms Comment Organism
TrmK
-
 Bacillus subtilis
tRNA: m1A22 methyltransferase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis