Cloned (Comment) | Organism |
---|---|
coexpression of catalytic subunit Trm61p and non-catalytic subunit Trm6p in Saccharomyces cerevisiae | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96FX7 and Q9UJA5 | Q96FX7: subunit Trm61p (catalytic subunit), Q9UJA5: subunit Trm6p (non-catalytic subunit) | - |
Purification (Comment) | Organism |
---|---|
coexpression of catalytic subunit Trm61p and non-catalytic subunit Trm6p in Saccharomyces cerevisiae, purification of the hTrm6p/hTrm61p complexes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in human tRNA3Lys | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methyladenine58 in human tRNA3Lys | - |
? | |
S-adenosyl-L-methionine + adenine58 in yeast initiator tRNAMet | no methylation of the A58U mutant of yeast initiator tRNA(Met) | Homo sapiens | S-adenosyl-L-homocysteine + N1-methyladenine58 in yeast initiator tRNAMet | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRm61p | catalytic subuni | Homo sapiens |
TRm6p | non-catalytic subunit | Homo sapiens |
tRNA m1A58 methyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | methylation of A58 is critical for maintaining the stability of initiator tRNAMet in yeast | Homo sapiens |