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Literature summary for 2.1.1.221 extracted from

  • Jackman, J.E.; Montange, R.K.; Malik, H.S.; Phizicky, E.M.
    Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9 (2003), RNA, 9, 574-585.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.221

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine9 in tRNA Saccharomyces cerevisiae TRM10 is required for the catalysis of at least 9 of the 10 m1G modifications observed (tRNA(Trp), tRNA(Pro), tRNA(Val), tRNAi(Met), tRNA(Ile), tRNAICG(Arg), and both tRNAUCU(Arg) species) at position 9 in yeast. It is likely that Trm10p is also responsible for modification of G9 of tRNAAla S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12400
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine9 in tRNA TRM10 is required for the catalysis of at least 9 of the 10 m1G modifications observed (tRNA(Trp), tRNA(Pro), tRNA(Val), tRNAi(Met), tRNA(Ile), tRNAICG(Arg), and both tRNAUCU(Arg) species) at position 9 in yeast. It is likely that Trm10p is also responsible for modification of G9 of tRNAAla Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA
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 ?

Synonyms

Synonyms Comment Organism
tRNA (guanine-N(1)-)-methyltransferase
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 Saccharomyces cerevisiae
tRNA m1G9 methyltransferase
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 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction guanine9 methylation activity is not detectable in trm10-DELTA/trm10-DELTA strain Saccharomyces cerevisiae