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Literature summary for 2.1.1.229 extracted from

  • Kalhor, H.R.; Clarke, S.
    Novel methyltransferase for modified uridine residues at the wobble position of tRNA (2003), Mol. Cell. Biol., 23, 9283-9292.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.229

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-Trm9 fusion protein in Escherichia coli DH5alpha cells Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P49957
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNAArg3
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
-
 ?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNAGlU
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
-
 ?

Synonyms

Synonyms Comment Organism
Trm9
-
 Saccharomyces cerevisiae
YML014w
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction in intact yeast cells, disruption of the TRM9 gene results in the complete loss of the modified wobble bases (5-methylcarbonylmethyluridine in tRNAArg3 and 5-methylcarbonylmethyl-2-thiouridine in tRNAGlu) and increased sensitivity at 37°C to paromomycin, a translational inhibitor. trm9-deletion mutants are hypersensitive to the translational inhibitor paromomycin at elevated temperatures, suggesting the importance of the methyl-esterified bases during heat shock Saccharomyces cerevisiae