Cloned (Comment) | Organism |
---|---|
expression of GST-Trm9 fusion protein in Escherichia coli DH5alpha cells | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P49957 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNAArg3 | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA | - |
? | |
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNAGlU | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trm9 | - |
Saccharomyces cerevisiae |
YML014w | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | in intact yeast cells, disruption of the TRM9 gene results in the complete loss of the modified wobble bases (5-methylcarbonylmethyluridine in tRNAArg3 and 5-methylcarbonylmethyl-2-thiouridine in tRNAGlu) and increased sensitivity at 37°C to paromomycin, a translational inhibitor. trm9-deletion mutants are hypersensitive to the translational inhibitor paromomycin at elevated temperatures, suggesting the importance of the methyl-esterified bases during heat shock | Saccharomyces cerevisiae |