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Literature summary for 2.1.1.229 extracted from

  • Fu, D.; Brophy, J.A.; Chan, C.T.; Atmore, K.A.; Begley, U.; Paules, R.S.; Dedon, P.C.; Begley, T.J.; Samson, L.D.
    Human AlkB homolog ABH8 is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival (2010), Mol. Cell. Biol., 30, 2449-2459.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.229

Cloned(Commentary)

Cloned (Comment) Organism
the FLAG-tagged-ABH8 coding region is cloned into the pBABEpuro or pBABEhygro retroviral vector. HeLa S3 cells are infected with retrovirus Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA Homo sapiens the enzyme catalyzes tRNA methylation to generate 5-methylcarboxymethyl uridine at the wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage survival S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96BT7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA the enzyme catalyzes tRNA methylation to generate 5-methylcarboxymethyl uridine at the wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage survival Homo sapiens S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
-
 ?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA ABH8 catalyzes RNA methylation through its methyltransferase domain. ABH8 MT domain alone exhibited weak methylase activity Homo sapiens S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA 5-(2-methoxy-2-oxoethyl)uridine i.e. 5-methoxycarboxymethyluridine ?

Synonyms

Synonyms Comment Organism
ABH8
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens DNA-damaging agents induce ABH8 expression in an ATM(central DNA damage kinase)-dependent manner down

General Information

General Information Comment Organism
malfunction ABH8 depletion in human cells reduces endogenous levels of 5-methoxycarboxymethyluridine in RNA and increases cellular sensitivity to DNA-damaging agents Homo sapiens
physiological function required for wobble uridine modification and DNA damage survival Homo sapiens