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Literature summary for 2.1.1.230 extracted from
- Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton (1981), J. Gen. Microbiol., 124, 291-297.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-homocysteine | - |
Streptomyces azureus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
S-adenosyl-L-methionine | pH 7.5, 35°C | Streptomyces azureus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | ionic optimum: 50-100 mM | Streptomyces azureus | |
| Mg2+ | ionic optimum: 5-20 mM | Streptomyces azureus | |
| NH4+ | ionic optimum: 50-100 mM | Streptomyces azureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | 38000 | gel filtration | Streptomyces azureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + adenosine1067 in 23S rRNA | Streptomyces azureus | the enzyme is involved in resistance to thiostrepton | S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces azureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces azureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + adenosine1067 in 23S rRNA | - |
Streptomyces azureus | S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenosine1067 in 23S rRNA | the enzyme is involved in resistance to thiostrepton | Streptomyces azureus | S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thiostrepton-resistance methylase | - |
Streptomyces azureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Streptomyces azureus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | quite active from 30°C-45°C | Streptomyces azureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 7.6 | - |
Streptomyces azureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.8 | pH 7.0: about 60% of maximal activity, pH 7.8: about 60% of maximal activity | Streptomyces azureus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
unstable below | Streptomyces azureus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
S-adenosyl-L-homocysteine | pH 7.5, 35°C | Streptomyces azureus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in resistance to thiostrepton | Streptomyces azureus |
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