Literature summary for 2.1.1.231 extracted from

Schroeder, G.; Wehinger, E.; Lukacin, R.; Wellmann, F.; Seefelder, W.; Schwab, W.; Schroeder, J.
Flavonoid methylation: a novel 4'-O-methyltransferase from Catharanthus roseus, and evidence that partially methylated flavanones are substrates of four different flavonoid dioxygenases (2004), Phytochemistry, 65, 1085-1094.

Search for more literature for 2.1.1.231

Cloned(Commentary)

Cloned (Comment)	Organism
gene CrOMT6, DNA and amino acid sequence determination and analysis, qualitative RT-PCR expression analysis, sequence comparison and phylogenetic tree, expression of His-tagged OMT6 in Escherichia coli	Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + 3'-O-methyleriodictyol	Catharanthus roseus	-	S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol	-	?

Organism

Organism	UniProt	Comment	Textmining
Catharanthus roseus	Q6VCW3	gene CrOMT6	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged OMT6 in Escherichia coli by nickel affinity chromatgraphy	Catharanthus roseus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	-	Catharanthus roseus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the only substrates found for CrOMT6 are 3'-O-methyl-eriodictyol (homoeriodictyol) and the corresponding flavones and flavonols, substrate specificty, overview. No or poor activity with naringenin, pentahydroxyflavanone, hesperetin, myricetin, 7,3'-O-dimethylquercetin, 7-O-methylquercetin, syringetin, apigenin, luteolin, tricetin, velutin, dihydrokaempferol, dihydroquercetin, dihydromyricetin, and 3'-O-methyl-dihydroquercetin, and also no activity with ferulic acid, coniferyl alcohol, vanillic acid vanillin, eugenol, isoeugenol, and guaicol. Not only the B-ring configuration, but also the size and shape of the A-ring are critical parts of the substrate specificity	Catharanthus roseus	?	-	?
S-adenosyl-L-methionine + 3'-O-methyleriodictyol	-	Catharanthus roseus	S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol	-	?
S-adenosyl-L-methionine + 3'-O-methyleriodictyol	-	Catharanthus roseus	S-adenosyl-L-homocysteine + 3',4'-O-dimethyleriodictyol	product identification by mass spectrometry	?
S-adenosyl-L-methionine + chrysoeriol	-	Catharanthus roseus	S-adenosyl-L-homocysteine + 4'-O-methylchrysoeriol	-	?
S-adenosyl-L-methionine + eriodictyol	-	Catharanthus roseus	S-adenosyl-L-homocysteine + 4'-O-methyleriodictyol	-	?
S-adenosyl-L-methionine + isorhamnetin	-	Catharanthus roseus	S-adenosyl-L-homocysteine + 4'-O-methylisorhamnetin	-	?
S-adenosyl-L-methionine + kaempferol	-	Catharanthus roseus	S-adenosyl-L-homocysteine + 4'-O-methylkaempferol	-	?
S-adenosyl-L-methionine + quercetin	-	Catharanthus roseus	S-adenosyl-L-homocysteine + 4'-O-methylquercetin	-	?

Synonyms

Synonyms	Comment	Organism
4'-O-methyltransferase	-	Catharanthus roseus
4'OMT	-	Catharanthus roseus
B-ring 4'OMT	-	Catharanthus roseus
flavonoid 4'-O-methyltransferase	UniProt	Catharanthus roseus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Catharanthus roseus

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Catharanthus roseus

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Release 2023.1 (January 2023)