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Literature summary for 2.1.1.246 extracted from

  • Opulencia, R.B.; Bose, A.; Metcalf, W.W.
    Physiology and posttranscriptional regulation of methanol:coenzyme M methyltransferase isozymes in Methanosarcina acetivorans C2A (2009), J. Bacteriol., 191, 6928-6935.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes mtaA1 and mtaA2, overexpression of N-terminally His6-tagged mtaA1 in Escherichia coli strain BL21(DE3), strains carrying various combinations of mtaC, mtaB, and mtaA expressed from the strong, tetracycline-regulated PmcrB(tetO1) promoter exhibit similar growth characteristics on methanol, showing that all combinations of MtaC, MtaB, and MtaA can form functional MT1/MT2 complexes. Variations in activity correlate with differences in protein abundance, despite the fact that all the encoding genes are expressed from the same promoter, overview. The mtaCBA transcripts show different stabilities, which are strongly influenced by the growth substrate, quantitative expression and activity analysis, overview Methanosarcina acetivorans

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans
-
genes mtaA1 and mtaA2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged mtaA1 in Escherichia coli strain BL21(DE3) by affinity chromatography Methanosarcina acetivorans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture cell growth on methanol, or trimethylamine, both with tetracycline Methanosarcina acetivorans
-
additional information the mtaCBA transcripts show different stabilities, which are strongly influenced by the growth substrate Methanosarcina acetivorans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activities of diverse mtaCBA transcripts from cells with different growth conditions, overview Methanosarcina acetivorans
0.5377
-
mtaC3B3A1 strain, cells grown on trimethylamine with tetracycline, pH 7.0, 37°C Methanosarcina acetivorans
0.5657
-
mtaC3B3A1 strain, cells grown on methanol with tetracycline, pH 7.0, 37°C Methanosarcina acetivorans

Synonyms

Synonyms Comment Organism
methanol-specific methyltransferase 2
-
Methanosarcina acetivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Methanosarcina acetivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Methanosarcina acetivorans

General Information

General Information Comment Organism
metabolism all combinations of MtaC, MtaB, and MtaA can form functional methanol-specific methyltransferase 1/2, MT1/MT2, complexes. Substrate-dependent, posttranscriptional regulation of mtaCBA operons, overview Methanosarcina acetivorans