Cloned (Comment) | Organism |
---|---|
genes mtaA1 and mtaA2, overexpression of N-terminally His6-tagged mtaA1 in Escherichia coli strain BL21(DE3), strains carrying various combinations of mtaC, mtaB, and mtaA expressed from the strong, tetracycline-regulated PmcrB(tetO1) promoter exhibit similar growth characteristics on methanol, showing that all combinations of MtaC, MtaB, and MtaA can form functional MT1/MT2 complexes. Variations in activity correlate with differences in protein abundance, despite the fact that all the encoding genes are expressed from the same promoter, overview. The mtaCBA transcripts show different stabilities, which are strongly influenced by the growth substrate, quantitative expression and activity analysis, overview | Methanosarcina acetivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | - |
genes mtaA1 and mtaA2 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged mtaA1 in Escherichia coli strain BL21(DE3) by affinity chromatography | Methanosarcina acetivorans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | cell growth on methanol, or trimethylamine, both with tetracycline | Methanosarcina acetivorans | - |
additional information | the mtaCBA transcripts show different stabilities, which are strongly influenced by the growth substrate | Methanosarcina acetivorans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activities of diverse mtaCBA transcripts from cells with different growth conditions, overview | Methanosarcina acetivorans |
0.5377 | - |
mtaC3B3A1 strain, cells grown on trimethylamine with tetracycline, pH 7.0, 37°C | Methanosarcina acetivorans |
0.5657 | - |
mtaC3B3A1 strain, cells grown on methanol with tetracycline, pH 7.0, 37°C | Methanosarcina acetivorans |
Synonyms | Comment | Organism |
---|---|---|
methanol-specific methyltransferase 2 | - |
Methanosarcina acetivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina acetivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanosarcina acetivorans |
General Information | Comment | Organism |
---|---|---|
metabolism | all combinations of MtaC, MtaB, and MtaA can form functional methanol-specific methyltransferase 1/2, MT1/MT2, complexes. Substrate-dependent, posttranscriptional regulation of mtaCBA operons, overview | Methanosarcina acetivorans |