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Literature summary for 2.1.1.246 extracted from
- Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression (1996), J. Biol. Chem., 271, 18725-18731.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes cmtA and cmtM, DNA and amino acid sequence determination and analysis, expressionin Escherichia coli strain XL-1 Blue | Methanosarcina barkeri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics of isozymes MT2-A and MT2-M, overview | Methanosarcina barkeri |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Methanosarcina barkeri |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, isozymes MT2-A and MT2-M, SDS-PAGE, x * 36700, about, isozyme MT2-A, sequence calculation | Methanosarcina barkeri |
| 36700 | - |
x * 34000, isozymes MT2-A and MT2-M, SDS-PAGE, x * 36700, about, isozyme MT2-A, sequence calculation | Methanosarcina barkeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M | Methanosarcina barkeri | - |
methyl-CoM + a [Co(I) methanol-specific corrinoid protein] | - |
r | |
| additional information | Methanosarcina barkeri | conversions of monomethylamine and dimethylamine to CH3-SCoM are dependent upon MT2-A, and are not supported by MT2-M. In contrast, MT2-M acts specifically in metabolism of methanol, but does not substitute for MT2-A in conversion of monomethylamine or dimethylamine. Nevertheless, both isozymes are capable of supporting the conversion of trimethylamine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | O30640 | isozymes MT2-A and MT2-M encoded by genes cmtA and cmtM | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | the isozymes MT2-A and MT2-M are differentially expressed depending upon the substrate available for growth | Methanosarcina barkeri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.154 | - |
MT2 activity in recombinant Escherichia coli cells transfected with gene cmtM, pH 7.2, 37°C | Methanosarcina barkeri |
| 1.21 | - |
MT2 activity in recombinant Escherichia coli cells transfected with gene cmtA, pH 7.2, 37°C | Methanosarcina barkeri |
| 2.2 | - |
MT2 activity in cells grown on trimethylamine, pH 7.2, 37°C | Methanosarcina barkeri |
| 7.5 | - |
MT2 activity in cells grown on methanol, pH 7.2, 37°C | Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a [methyl-Co(III) methanol-specific corrinoid protein] + 3-mercaptopropanoic acid | - |
Methanosarcina barkeri | methyl-3-mercaptopropanoic acid + a [Co(I) methanol-specific corrinoid protein] | - |
r | |
| a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M | - |
Methanosarcina barkeri | methyl-CoM + a [Co(I) methanol-specific corrinoid protein] | - |
r | |
| a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M | the enzyme methylcobamide:CoM methyltransferase, MT2, catalyzes the transfer of the methyl group from the MT1-bound methylcobamide prosthetic group to coenzyme M | Methanosarcina barkeri | methyl-CoM + a [Co(I) methanol-specific corrinoid protein] | - |
r | |
| additional information | conversions of monomethylamine and dimethylamine to CH3-SCoM are dependent upon MT2-A, and are not supported by MT2-M. In contrast, MT2-M acts specifically in metabolism of methanol, but does not substitute for MT2-A in conversion of monomethylamine or dimethylamine. Nevertheless, both isozymes are capable of supporting the conversion of trimethylamine | Methanosarcina barkeri | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, isozymes MT2-A and MT2-M, SDS-PAGE, x * 36700, about, isozyme MT2-A, sequence calculation | Methanosarcina barkeri |
| More | the isozymes MT2-A and MT2-M differ in their overall charge | Methanosarcina barkeri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylcobamide:coenzyme M methyltransferase | - |
Methanosarcina barkeri |
| methylcobamide:CoM methyltransferase | - |
Methanosarcina barkeri |
| MT2-A | - |
Methanosarcina barkeri |
| MT2-M | - |
Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Methanosarcina barkeri |
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