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Literature summary for 2.1.1.249 extracted from
- Purification and characterization of dimethylamine: 5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro (1998), Eur. J. Biochem., 253, 692-697.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | acts as a powerful allosteric effector on the methyltransferase reaction. ATP serves as the substrate for MAP, which, after being phosphorylated by ATP, operates in the reductive activation of the corrinoid methyltransferase | Methanosarcina barkeri |  |
| methyltransferase activating protein | MAP, dependent on. ATP serves as the substrate for MAP, which, after being phosphorylated by ATP, operates in the reductive activation of the corrinoid methyltransferase | Methanosarcina barkeri | |
| additional information | as isolated, DMA-MT is inactive, but it can enzymically be reactivated by methyltransferase activating protein, ATP, and hydrogenase | Methanosarcina barkeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Monomethylamine | competitive inhibitor | Methanosarcina barkeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Methanosarcina barkeri |
| 100000 | - |
gel filtration | Methanosarcina barkeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | Methanosarcina barkeri Fusaro / DSM 804 | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
DSM 804 | - |
| Methanosarcina barkeri Fusaro / DSM 804 | - |
DSM 804 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 62.8fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and anion exchange chromatography | Methanosarcina barkeri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:trimethylamine-grown cell | - |
Methanosarcina barkeri | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | methylation of the corrinoid prosthetic group | Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri Fusaro / DSM 804 | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | methylation of the corrinoid prosthetic group | Methanosarcina barkeri Fusaro / DSM 804 | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, SDS-PAGE | Methanosarcina barkeri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dimethyl-12-HBI methyltransferase | - |
Methanosarcina barkeri |
| dimethylamine/5-hydroxybenzimidazolylcobamide methyltransferase | - |
Methanosarcina barkeri |
| DMA-MT | - |
Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
- |
Methanosarcina barkeri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 46 | rapid decline of activity above 46°C, inactive at 55°C | Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
- |
Methanosarcina barkeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| corrinoid protein | prosthetic group, with a corrinoid content of 0.9 mol B12/mol holoenzyme | Methanosarcina barkeri | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
Monomethylamine | pH 7.0, 37°C | Methanosarcina barkeri | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methanosarcina barkeri | isoelectric focusing | - |
4.7 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | as-isolated DMA-MT is inactive. The enzyme can be reactivated to yield the methylated B12-HBI prosthetic group by a system that comprises a methyl donor, a reductant, ATP and one (or more) component(s) present in the partially purified methyltransferase activating protein, MAP, fraction | Methanosarcina barkeri |
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