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Literature summary for 2.1.1.250 extracted from
- The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons (2000), J. Bacteriol., 182, 2520-2529.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mttB, co-transcription with gene mtbB1 encoding the DMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes of all methyltransferases each contain a single in-frame amber codon | Methanosarcina barkeri |
| gene mttB, co-transcription with gene mtbB1 encoding the DMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes of all methyltransferases each contain a single in-frame amber codon | Methanosarcina thermophila |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the determined N-terminus of MttB, encoded by mttB, shows a UAG codon, which is represented in both strands of the DNA, follows at codon position 334. Cessation of translation at the UAG codon results in a 34-kDa product rather than the abundant 53-kDa polypeptide purified from TMA-grown cells | Methanosarcina barkeri |
| additional information | the determined N-terminus of MttB, encoded by mttB, shows a UAG codon, which is represented in both strands of the DNA, follows at codon position 334. Cessation of translation at the UAG codon results in a 34-kDa product rather than the abundant 53-kDa polypeptide purified from TMA-grown cells | Methanosarcina thermophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? |  |
| trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | Methanosarcina thermophila | - |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | O93658 | MttB; single copy gene mttB | - |
| Methanosarcina thermophila | Q9P995 | MttB; gene mttB | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
| trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | - |
Methanosarcina thermophila | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
| trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | the TMA methyltransferase cognate corrinoid protein MttC | Methanosarcina barkeri | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
| trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | the TMA methyltransferase cognate corrinoid protein MttC | Methanosarcina thermophila | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MttB | - |
Methanosarcina barkeri |
| MttB | - |
Methanosarcina thermophila |
| TMA methyltransferase | - |
Methanosarcina barkeri |
| TMA methyltransferase | - |
Methanosarcina thermophila |
| trimethylamine methyltransferase | - |
Methanosarcina barkeri |
| trimethylamine methyltransferase | - |
Methanosarcina thermophila |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | three different methyltransferases initiate methanogenesis from trimethylamine, dimethylamine, or monomethylamine by methylating different cognate corrinoid proteins that are subsequently used to methylate coenzyme M | Methanosarcina barkeri |
| metabolism | three different methyltransferases initiate methanogenesis from trimethylamine, dimethylamine, or monomethylamine by methylating different cognate corrinoid proteins that are subsequently used to methylate coenzyme M | Methanosarcina thermophila |
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