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Literature summary for 2.1.1.253 extracted from
- Tetramethylammonium:coenzyme M methyltransferase system from Methanococcoides sp. (1998), Arch. Microbiol., 170, 220-226.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ti(III) citrate | dependent on | Methanosarcina barkeri |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Methanosarcina barkeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Methanosarcina barkeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
gene mtqA | - |
| Methanosarcina barkeri NaT1 | - |
gene mtqA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ultracentrifugation, a first step of anion exchange chromatography, hydroxyapatite chromatography, a second step of anion exchange chromatography, adsorption and again a third step of anion exchange chromatography | Methanosarcina barkeri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:tetramethylammonium-grown cell | - |
Methanosarcina barkeri | - |
| additional information | cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity | Methanosarcina barkeri | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Tetramethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation | Methanosarcina barkeri | ? | - |
? | |
| additional information | the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Tetramethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation | Methanosarcina barkeri NaT1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Methanosarcina barkeri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MtqA | - |
Methanosarcina barkeri |
| tetramethylammonium:coenzyme M methyltransferase | - |
Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Methanosarcina barkeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Methanosarcina barkeri | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 | Methanosarcina barkeri |
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