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Literature summary for 2.1.1.255 extracted from
- Biochemistry and molecular genetics of the biosynthesis of the earthy odorant methylisoborneol in Streptomyces coelicolor (2008), J. Am. Chem. Soc., 130, 8908-8909.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Streptomyces coelicolor |
| gene sco7700, DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, Michaelis-Menten kinetics | Streptomyces coelicolor | |
| 0.0043 | - |
S-adenosyl-L-methionine | pH 7.0, 37°C, recombinant enzyme | Streptomyces coelicolor |  |
| 0.0043 | - |
S-adenosyl-L-methionine | pH and temperature not specified in the publication | Streptomyces coelicolor | |
| 0.0131 | - |
geranyl diphosphate | pH 7.0, 37°C, recombinant enzyme | Streptomyces coelicolor | |
| 0.0131 | - |
geranyl diphosphate | pH and temperature not specified in the publication | Streptomyces coelicolor | |
| 0.026 | - |
(E)-2-methylgeranyl diphosphate | pH 7.0, 37°C, recombinant enzyme | Streptomyces coelicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49937 | - |
x * 49937, recombinant N-terminally His6-tagged soluble SCO7700, mass spectrometry, x * 49955, sequence calculation | Streptomyces coelicolor |
| 49955 | - |
x * 49937, recombinant N-terminally His6-tagged soluble SCO7700, mass spectrometry, x * 49955, sequence calculation | Streptomyces coelicolor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + geranyl diphosphate | Streptomyces coelicolor | - |
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | Q9F1Y5 | - |
- |
| Streptomyces coelicolor | Q9F1Y5 | gene sco7700 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged soluble SCO7700 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity and over 95% purity | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | SCO7701 shows no activity with with farnesyl diphosphate or geranylgeranyl diphosphate | Streptomyces coelicolor | ? | - |
? | |
| S-adenosyl-L-methionine + (E)-2-methylgeranyl diphosphate | - |
Streptomyces coelicolor | S-adenosyl-L-homocysteine + 2-methylisoborneol | - |
? | |
| S-adenosyl-L-methionine + geranyl diphosphate | - |
Streptomyces coelicolor | S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate | - |
? | |
| S-adenosyl-L-methionine + geranyl diphosphate | - |
Streptomyces coelicolor | S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate | GC-MS analysis of the products from recombinant SCO7700 reveals the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene at 6%, beta-pinene at 23%, limonene at 32%, gamma-terpinene at 29%, and delta-terpinene at 10% accompanied by traces of the monoterpene alcohols beta-terpineol, 4-terpineol, and alpha-terpineol | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49937, recombinant N-terminally His6-tagged soluble SCO7700, mass spectrometry, x * 49955, sequence calculation | Streptomyces coelicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C-methyl transferase | - |
Streptomyces coelicolor |
| SCO7701 | - |
Streptomyces coelicolor |
| SCO7701 protein | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptomyces coelicolor |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0075 | - |
S-adenosyl-L-methionine | pH 7.0, 37°C, recombinant enzyme | Streptomyces coelicolor | |
| 0.0075 | - |
geranyl diphosphate | pH 7.0, 37°C, recombinant enzyme | Streptomyces coelicolor | |
| 0.0075 | - |
S-adenosyl-L-methionine | pH and temperature not specified in the publication | Streptomyces coelicolor | |
| 0.0075 | - |
geranyl diphosphate | pH and temperature not specified in the publication | Streptomyces coelicolor | |
| 0.039 | - |
(E)-2-methylgeranyl diphosphate | pH 7.0, 37°C, recombinant enzyme | Streptomyces coelicolor | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Streptomyces coelicolor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Streptomyces coelicolor | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the C-methyl transferase SCO7701 catalyzes the two-step conversion of geranyl diphosphate to 2-methyl-isoborneol by way of 2-methylgeranyl diphosphate | Streptomyces coelicolor |
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