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Literature summary for 2.1.1.260 extracted from

  • Schilling, V.; Peifer, C.; Buchhaupt, M.; Lamberth, S.; Lioutikov, A.; Rietschel, B.; Koetter, P.; Entian, K.D.
    Genetic interactions of yeast NEP1 (EMG1), encoding an essential factor in ribosome biogenesis (2012), Yeast, 29, 167-183.
    View publication on PubMed
Search for more literature for 2.1.1.260

Protein Variants

Protein Variants Comment Organism
additional information lethal phenotype of a DELTAnep1 deletion, overexpression of RPS19B and also deletions within genes DELTAnop6 and DELTAtma23, respectively, which both encode small, positively charged fungal-specific proteins, suppress the DELTAnep1 growth deficiency. Identification of DELTanep1-specific genetic interactions, polysome profiles and phenotypes, overview. DELTAAutp30 enhances the phenotype of DELTAnep1 and is a multi-copy suppressor Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA Saccharomyces cerevisiae
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 S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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 strains from BY strain background or isogenic to strain CEN.PK2, gene nep1
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Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from subcellular fractions by sucrose density gradient fractionation Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
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 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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 ?

Synonyms

Synonyms Comment Organism
Emg1
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 Saccharomyces cerevisiae
Nep1
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 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction lethal phenotype of a DELTAnep1 deletion, deletions in ribosome quality and functional control genes lead to DELTAnep1 growth deficiency. Except for DELTArps18b, deletions in the identified ribosome biogenesis genes are synthetically lethal with DELTAnep1. The DELTAutp30 deletion itself has no phenotype but it enforces all nep1-1ts mutant phenotypes, utp30 overexpression partially restores the nep1-1ts growth deficiency Saccharomyces cerevisiae
metabolism Nep1 interacting genes correspond to ribosome biogenesis, i.e. RPS18A, RPS18B, RRP8, EFG1, UTP30, to ribosome quality control, i.e. UBP3, BRE5, UBP6, and to ribosome functional control, i.e. DOM34, no-go decay Saccharomyces cerevisiae
additional information genome-wide yeast screen to uncover synthetic interactions of DELTAnep1 Saccharomyces cerevisiae
physiological function the nucleolar essential protein Nep1 is an important trans-acting factor in the 90S preribosome. Nep1 methylates the hypermodified psi1191 base of 18S rRNA and has an additional essential function during ribosome biogenesis, i.e. in 40S subunit synthesis. Utp30 and Nep1 act together during pre-ribosomal complex formation and, along with Rps18, provide the surface for the Rps19 assembly to the 90S pre-ribosome Saccharomyces cerevisiae