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Literature summary for 2.1.1.267 extracted from

  • Cacace, S.; Schröder, G.; Wehinger, E.; Strack, D.; Schmidt, J.; Schröder, J.
    A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations (2003), Phytochemistry, 62, 127-137.
    View publication on PubMed
Search for more literature for 2.1.1.267

Cloned(Commentary)

Cloned (Comment) Organism
-
 Catharanthus roseus
gene CrOMT2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression as His-tagged enzyme in Escherichia coli Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 SDS-PAGE Catharanthus roseus
39145
-
 x * 39145, sequence calculation Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Catharanthus roseus CROMT2 is involved in biosynthesis of glycosides and anthocyanins ?
-
 ?
additional information Catharanthus roseus OMT2 performs two sequential methylations at the 3'-and 5'-positions of the B-ring in myricetin (flavonol) and dihydromyricetin (dihydroflavonol) ?
-
 ?
S-adenosyl-L-methionine + myricetin Catharanthus roseus involvement in biosynthesis of flavonol glycosides and anthocyanins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-
Catharanthus roseus
-
 Madagascar periwinkle
-
Catharanthus roseus Q8GSN1 gene CrOMT2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Catharanthus roseus
recombinant His-tagged OMT2 by nickel affinity chromatography Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Catharanthus roseus
-
cell suspension culture dark-grown Catharanthus roseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000219
-
-
 Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CROMT2 is involved in biosynthesis of glycosides and anthocyanins Catharanthus roseus ?
-
 ?
additional information OMT2 performs two sequential methylations at the 3'-and 5'-positions of the B-ring in myricetin (flavonol) and dihydromyricetin (dihydroflavonol) Catharanthus roseus ?
-
 ?
additional information no activity with kaempferol, dihydrokaempferol, caffeate and caffeate esters Catharanthus roseus ?
-
 ?
S-adenosyl-L-methionine + 2,3-dihydromyricetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 3'-O-methyl-2,3-dihydromyricetin
-
 ?
S-adenosyl-L-methionine + 3'-O-methyl-2,3-dihydromyricetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 3',5'-di-O-methyl-2,3-dihydromyricetin i.e. syringetin ?
S-adenosyl-L-methionine + 3'-O-methyl-2,3-dihydroquercetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 3',4'-di-O-methyl-2,3-dihydroquercetin
-
 ?
S-adenosyl-L-methionine + 3'-O-methylmyricetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 3',5'-di-O-methylmyricetin
-
 ?
S-adenosyl-L-methionine + 3'-O-methylquercetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 3',4'-di-O-methylquercetin
-
 ?
S-adenosyl-L-methionine + dihydromyricetin two sequential methylations at the 3'- and 5'-positions of the B-ring in dihydromyricetin Catharanthus roseus ?
-
 ?
S-adenosyl-L-methionine + dihydromyricetin 54% of the activity with myricetin Catharanthus roseus S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + dihydroquercetin 22% of the activity with myricetin Catharanthus roseus S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + dihydroquercetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 3'-O-methyl-2,3-dihydroquercetin
-
 ?
S-adenosyl-L-methionine + myricetin two sequential methylations at the 3'- and 5'-positions of the B-ring in myricetin, activity is strictly confined to flavonols and dihydroflavonols, it requires at least 2 B-ring hydroxyl groups Catharanthus roseus ?
-
 ?
S-adenosyl-L-methionine + myricetin involvement in biosynthesis of flavonol glycosides and anthocyanins Catharanthus roseus ?
-
 ?
S-adenosyl-L-methionine + myricetin
-
 Catharanthus roseus S-adenosyl-L-homocysteine + syringetin
-
 ?
S-adenosyl-L-methionine + myricetin best substrate Catharanthus roseus S-adenosyl-L-homocysteine + 3'-O-methylmyricetin
-
 ?
S-adenosyl-L-methionine + quercetin low activity Catharanthus roseus S-adenosyl-L-homocysteine + 3'-O-methylquercetin
-
 ?

Subunits

Subunits Comment Organism
? x * 39145, sequence calculation Catharanthus roseus

Synonyms

Synonyms Comment Organism
CrOMT2
-
 Catharanthus roseus
OMT2
-
 Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Catharanthus roseus

General Information

General Information Comment Organism
metabolism CrOMT2 is involved in the biosynthesis of flavonol glycosides and anthocyanins in Catharanthus roeus Catharanthus roseus