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Literature summary for 2.1.1.267 extracted from
- Cation dependent O-methyltransferases from rice (2008), Planta, 227, 641-647.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as glutathione S-transferase fusion protein | Oryza sativa |
| isoform ROMT-15, expression in Escherichia coli | Oryza sativa |
| isoform ROMT-17, expression in Escherichia coli | Oryza sativa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D168L | isoform ROMT-15, complete loss of activity | Oryza sativa |
| D168L | mutation abolishes activity (with quercetin as substrate) | Oryza sativa |
| D194L | isoform ROMT-15, complete loss of activity | Oryza sativa |
| D194L | mutation abolishes activity (with quercetin as substrate) | Oryza sativa |
| D209L | isoform ROMT-17, complete loss of activity | Oryza sativa |
| D209L | mutation abolishes activity (with quercetin as substrate) | Oryza sativa |
| D234L | isoform ROMT-17, complete loss of activity | Oryza sativa |
| D234L | mutation abolishes activity (with quercetin as substrate) | Oryza sativa |
| E112L | isoform ROMT-17, 14% loss of activity | Oryza sativa |
| E112L | mutation results in 40% loss of activity (with quercetin as substrate) | Oryza sativa |
| E69L | isoform ROMT-15, 14% loss of activity | Oryza sativa |
| E69L | mutation results in 14% loss of activity (with quercetin as substrate) | Oryza sativa |
| N195I | isoform ROMT-15, complete loss of activity | Oryza sativa |
| N195I | mutation abolishes activity (with quercetin as substrate) | Oryza sativa |
| N235I | isoform ROMT-17, complete loss of activity | Oryza sativa |
| N235I | mutation abolishes activity (with quercetin as substrate) | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
tricetin | isoform ROMT-17 | Oryza sativa |  |
| 0.044 | - |
myricetin | pH 7.5, 37°C | Oryza sativa | |
| 0.044 | - |
myricetin | isoform ROMT-17 | Oryza sativa | |
| 0.072 | - |
tricetin | isoform ROMT-15 | Oryza sativa | |
| 0.072 | - |
caffeoyl-coenzyme A | isoform ROMT-17 | Oryza sativa | |
| 0.076 | - |
caffeoyl-coenzyme A | isoform ROMT-15 | Oryza sativa | |
| 0.08 | - |
myricetin | pH 7.5, 37°C | Oryza sativa | |
| 0.08 | - |
myricetin | isoform ROMT-15 | Oryza sativa | |
| 0.09 | - |
luteolin | isoform ROMT-15 | Oryza sativa | |
| 0.104 | - |
luteolin | isoform ROMT-17 | Oryza sativa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | isoform ROMT-15, poorly substitutes for Mg2+ | Oryza sativa | |
| Ca2+ | isoform ROMT-17, poorly substitutes for Mg2+ | Oryza sativa | |
| Co2+ | isoform ROMT-15, may substitute for Mg2+ | Oryza sativa | |
| Co2+ | isoform ROMT-17, may substitute for Mg2+ | Oryza sativa | |
| Co2+ | the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Co2+ shows 41% of the activity with Mg2+ (with quercetin as substrate) | Oryza sativa | |
| Co2+ | the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Co2+ shows 82% of the activity with Mg2+ (with quercetin as substrate) | Oryza sativa | |
| Mg2+ | isoform ROMT-15, required | Oryza sativa | |
| Mg2+ | isoform ROMT-17, required | Oryza sativa | |
| Mg2+ | the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity (with quercetin as substrate) | Oryza sativa | |
| Mn2+ | the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Mn2+ shows 69% of the activity with Mg2+ (with quercetin as substrate) | Oryza sativa | |
| Mn2+ | the enzyme is metal-dependent, Mg2+ is the best cation for catalytic activity, Mn2+ shows 74% of the activity with Mg2+ (with quercetin as substrate) | Oryza sativa | |
| Zn2+ | isoform ROMT-15, poorly substitutes for Mg2+ | Oryza sativa | |
| Zn2+ | isoform ROMT-17, poorly substitutes for Mg2+ | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27800 | - |
x * 27800, calculated, isoform ROMT-15 | Oryza sativa |
| 30600 | - |
x * 30600, SDS-PAGE, glutathione S-transferase fusion protein | Oryza sativa |
| 31100 | - |
x * 31100, SDS-PAGE, glutathione S-transferase fusion protein | Oryza sativa |
| 32000 | - |
x * 32000, calculated, isoform ROMT-17 | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
| Oryza sativa | - |
isoform ROMT-15 | - |
| Oryza sativa | - |
isoform ROMT-17 | - |
| Oryza sativa | Q9XGP7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | isoform ROMT-15, expression in all tissues investigated | Oryza sativa | - |
| root | - |
Oryza sativa | - |
| root | isoform ROMT-15, highest expression in root and stem | Oryza sativa | - |
| stem | - |
Oryza sativa | - |
| stem | isoform ROMT-15, highest expression in root and stem | Oryza sativa | - |
| stem | expressed in stems only | Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 S-adenosyl-L-methionine + myricetin | the enzyme also catalyzes the methylation of luteolin, tricetin and caffeoyl-CoA. ROMT-15 exhibits similar kcat/Km values for the four substrates. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity | Oryza sativa | 2 S-adenosyl-L-homocysteine + syringetin | syringetin is the 3',5'-dimethyl ether of myricetin | ? | |
| 2 S-adenosyl-L-methionine + myricetin | the enzyme also catalyzes the methylation of luteolin, tricetin and caffeoyl-CoA. ROMT-17 prefers tricetin. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity | Oryza sativa | 2 S-adenosyl-L-homocysteine + syringetin | syringetin is the 3',5'-dimethyl ether of myricetin | ? | |
| additional information | isoform ROMT-15, no substrate: naringenin, apigenin, kaempferol | Oryza sativa | ? | - |
? | |
| additional information | isoform ROMT-17, no substrate: naringenin, apigenin, kaempferol | Oryza sativa | ? | - |
? | |
| S-adenosyl-L-methionine + 7,8-dihydroxyflavone | - |
Oryza sativa | S-adenosyl-L-homocysteine + ? | - |
? | |
| S-adenosyl-L-methionine + caffeoyl-coenzyme A | isoform ROMT-15, 100% of the activity with myricetin | Oryza sativa | S-adenosyl-L-homocysteine + ? | - |
? | |
| S-adenosyl-L-methionine + caffeoyl-coenzyme A | isoform ROMT-17, 100% of the activity with myricetin | Oryza sativa | S-adenosyl-L-homocysteine + ? | - |
? | |
| S-adenosyl-L-methionine + luteolin | isoform ROMT-15, 92% of the activity with myricetin | Oryza sativa | S-adenosyl-L-homocysteine + ? | isoform ROMT-15, methylation at 3'-hydroxyl group | ? | |
| S-adenosyl-L-methionine + luteolin | isoform ROMT-17, 92% of the activity with myricetin | Oryza sativa | S-adenosyl-L-homocysteine + ? | isoform ROMT-17, methylation at 3'-hydroxyl group | ? | |
| S-adenosyl-L-methionine + myricetin | - |
Oryza sativa | S-adenosyl-L-homocysteine + syringetin | isoform ROMT-15, methylation at 3'-hydroxyl group and at the 5' hydroxyl group | ? | |
| S-adenosyl-L-methionine + myricetin | - |
Oryza sativa | S-adenosyl-L-homocysteine + syringetin | isoform ROMT-17, methylation at 3'-hydroxyl group and at the 5' hydroxyl group | ? | |
| S-adenosyl-L-methionine + quercetin | - |
Oryza sativa | S-adenosyl-L-homocysteine + ? | isoform ROMT-15, methylation at 3'-hydroxyl group | ? | |
| S-adenosyl-L-methionine + quercetin | - |
Oryza sativa | S-adenosyl-L-homocysteine + ? | isoform ROMT-17, methylation at 3'-hydroxyl group | ? | |
| S-adenosyl-L-methionine + tricetin | isoform ROMT-15, methylation at 3'-hydroxyl group and at the 5' hydroxyl group. 87% of the activity with myricetin | Oryza sativa | S-adenosyl-L-homocysteine + ? | - |
? | |
| S-adenosyl-L-methionine + tricetin | isoform ROMT-17, methylation at 3'-hydroxyl group and at the 5' hydroxyl group. 87% of the activity with myricetin | Oryza sativa | S-adenosyl-L-homocysteine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27800, calculated, isoform ROMT-15 | Oryza sativa |
| ? | x * 32000, calculated, isoform ROMT-17 | Oryza sativa |
| ? | x * 30600, SDS-PAGE, glutathione S-transferase fusion protein | Oryza sativa |
| ? | x * 31100, SDS-PAGE, glutathione S-transferase fusion protein | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ROMT-15 | - |
Oryza sativa |
| ROMT-17 | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Oryza sativa | |
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