Cloned (Comment) | Organism |
---|---|
gene OMT, DNA and amino acid sequence determination and analysis, phylogenetic analysis, gene OMT shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes, encoding dihydroflavonol reductase (DFR), flavonoid-3',5'-hydroxylase (F3'5'H), and anthocyanidin synthase (ANS) | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | silencing of AnthOMT in hypocotyl tissue, several hypocotyls of three lines (57, 85 and 87) show an anthocyanin profile that is different to that of the wild-type, and show a strong reduction in malvidin-type anthocyanins, while other lines (64, 66 and 71) show only wild-type anthocyanin profiles | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
delphinidin 3-O-beta-D-glucoside | pH 7.0, 30°C | Solanum lycopersicum | |
0.1 | - |
quercetin | pH 7.0, 30°C | Solanum lycopersicum | |
0.437 | - |
quercetin 3-O-beta-D-glucoside | pH 7.0, 30°C | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26300 | - |
x * 26300, SDS-PAGE | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum lycopersicum | the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
var. MoneyMaker, gene OMT | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Solanum lycopersicum | - |
fruit | - |
Solanum lycopersicum | - |
hypocotyl | - |
Solanum lycopersicum | - |
additional information | the OMT gene shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes | Solanum lycopersicum | - |
root | - |
Solanum lycopersicum | - |
seedling | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred | Solanum lycopersicum | ? | - |
? | |
additional information | the specificity of AnthOMT is mainly directed towards the 3' and 5' positions of the anthocyanin B-ring, to produce petunidin and malvidin glucosides, and is not active at the 4' position, substrate specificity of enzyme AnthOMT, overview. Poor activity with caffeic acid, no activity with pelargonidin-3-glucoside and delphinidin. AnthOMT is able to methylate the more complex substrates, e.g. an an extract of semi-polar compounds from Ros/Del tomato fruits preparation, resulting in a strong increase in malvidin 3-(4-coumaroyl)-rutinoside-5-glucoside | Solanum lycopersicum | ? | - |
? | |
S-adenosyl-L-methionine + cyanidin 3-O-beta-D-glucoside | - |
Solanum lycopersicum | S-adenosyl-L-homocysteine + peonidin 3-O-beta-D-glucoside | - |
? | |
S-adenosyl-L-methionine + delphinidin 3-O-beta-D-glucoside | high activity | Solanum lycopersicum | S-adenosyl-L-homocysteine + petunidin 3-O-beta-D-glucoside | - |
? | |
S-adenosyl-L-methionine + myricitin | - |
Solanum lycopersicum | S-adenosyl-L-homocysteine + laricitrin | two product isomers | ? | |
S-adenosyl-L-methionine + quercetin | - |
Solanum lycopersicum | S-adenosyl-L-homocysteine + isorhamnetin | - |
? | |
S-adenosyl-L-methionine + quercetin 3-O-beta-D-glucoside | - |
Solanum lycopersicum | S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-glucoside | - |
? | |
S-adenosyl-L-methionine + quercetin 3-O-beta-D-rutinoside | - |
Solanum lycopersicum | S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-rutinoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26300, SDS-PAGE | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
AnthOMT | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | - |
Solanum lycopersicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
quercetin 3-O-beta-D-glucoside | pH 7.0, 30°C | Solanum lycopersicum | |
0.02 | - |
quercetin | pH 7.0, 30°C | Solanum lycopersicum | |
0.025 | - |
delphinidin 3-O-beta-D-glucoside | pH 7.0, 30°C | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | loss of activity at pH 5.0-6.0, inactive below | Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
malfunction | downregulation of AnthOMT in planta decreases the representation of methylated anthocyanins in tomato tissues as well as overall anthocyanin accumulation | Solanum lycopersicum |
physiological function | role of the enzyme in the diversification of tomato anthocyanins | Solanum lycopersicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
quercetin 3-O-beta-D-glucoside | pH 7.0, 30°C | Solanum lycopersicum | |
0.2 | - |
quercetin | pH 7.0, 30°C | Solanum lycopersicum | |
2.08 | - |
delphinidin 3-O-beta-D-glucoside | pH 7.0, 30°C | Solanum lycopersicum |