Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.267 extracted from

  • Gomez Roldan, M.V.; Outchkourov, N.; van Houwelingen, A.; Lammers, M.; Romero de la Fuente, I.; Ziklo, N.; Aharoni, A.; Hall, R.D.; Beekwilder, J.
    An O-methyltransferase modifies accumulation of methylated anthocyanins in seedlings of tomato (2014), Plant J., 80, 695-708.
    View publication on PubMed
Search for more literature for 2.1.1.267

Cloned(Commentary)

Cloned (Comment) Organism
gene OMT, DNA and amino acid sequence determination and analysis, phylogenetic analysis, gene OMT shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes, encoding dihydroflavonol reductase (DFR), flavonoid-3',5'-hydroxylase (F3'5'H), and anthocyanidin synthase (ANS) Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
additional information silencing of AnthOMT in hypocotyl tissue, several hypocotyls of three lines (57, 85 and 87) show an anthocyanin profile that is different to that of the wild-type, and show a strong reduction in malvidin-type anthocyanins, while other lines (64, 66 and 71) show only wild-type anthocyanin profiles Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 delphinidin 3-O-beta-D-glucoside pH 7.0, 30°C Solanum lycopersicum
0.1
-
 quercetin pH 7.0, 30°C Solanum lycopersicum
0.437
-
 quercetin 3-O-beta-D-glucoside pH 7.0, 30°C Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26300
-
 x * 26300, SDS-PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred ?
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
 var. MoneyMaker, gene OMT
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Solanum lycopersicum
-
fruit
-
 Solanum lycopersicum
-
hypocotyl
-
 Solanum lycopersicum
-
additional information the OMT gene shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes Solanum lycopersicum
-
root
-
 Solanum lycopersicum
-
seedling
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred Solanum lycopersicum ?
-
 ?
additional information the specificity of AnthOMT is mainly directed towards the 3' and 5' positions of the anthocyanin B-ring, to produce petunidin and malvidin glucosides, and is not active at the 4' position, substrate specificity of enzyme AnthOMT, overview. Poor activity with caffeic acid, no activity with pelargonidin-3-glucoside and delphinidin. AnthOMT is able to methylate the more complex substrates, e.g. an an extract of semi-polar compounds from Ros/Del tomato fruits preparation, resulting in a strong increase in malvidin 3-(4-coumaroyl)-rutinoside-5-glucoside Solanum lycopersicum ?
-
 ?
S-adenosyl-L-methionine + cyanidin 3-O-beta-D-glucoside
-
 Solanum lycopersicum S-adenosyl-L-homocysteine + peonidin 3-O-beta-D-glucoside
-
 ?
S-adenosyl-L-methionine + delphinidin 3-O-beta-D-glucoside high activity Solanum lycopersicum S-adenosyl-L-homocysteine + petunidin 3-O-beta-D-glucoside
-
 ?
S-adenosyl-L-methionine + myricitin
-
 Solanum lycopersicum S-adenosyl-L-homocysteine + laricitrin two product isomers ?
S-adenosyl-L-methionine + quercetin
-
 Solanum lycopersicum S-adenosyl-L-homocysteine + isorhamnetin
-
 ?
S-adenosyl-L-methionine + quercetin 3-O-beta-D-glucoside
-
 Solanum lycopersicum S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-glucoside
-
 ?
S-adenosyl-L-methionine + quercetin 3-O-beta-D-rutinoside
-
 Solanum lycopersicum S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-rutinoside
-
 ?

Subunits

Subunits Comment Organism
? x * 26300, SDS-PAGE Solanum lycopersicum

Synonyms

Synonyms Comment Organism
AnthOMT
-
 Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 quercetin 3-O-beta-D-glucoside pH 7.0, 30°C Solanum lycopersicum
0.02
-
 quercetin pH 7.0, 30°C Solanum lycopersicum
0.025
-
 delphinidin 3-O-beta-D-glucoside pH 7.0, 30°C Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
5 9 loss of activity at pH 5.0-6.0, inactive below Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Solanum lycopersicum

General Information

General Information Comment Organism
malfunction downregulation of AnthOMT in planta decreases the representation of methylated anthocyanins in tomato tissues as well as overall anthocyanin accumulation Solanum lycopersicum
physiological function role of the enzyme in the diversification of tomato anthocyanins Solanum lycopersicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.032
-
 quercetin 3-O-beta-D-glucoside pH 7.0, 30°C Solanum lycopersicum
0.2
-
 quercetin pH 7.0, 30°C Solanum lycopersicum
2.08
-
 delphinidin 3-O-beta-D-glucoside pH 7.0, 30°C Solanum lycopersicum