Cloned (Comment) | Organism |
---|---|
BAMT cDNA expressed in Escherichia coli | Clarkia breweri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
SDS-PAGE | Clarkia breweri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + benzoate | Clarkia breweri | emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity | S-adenosyl-L-homocysteine + methyl benzoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clarkia breweri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from 5- to 10-day-old upper and lower petal lobes by DE53 anion exchange, phenylSepharose 6FF and MonoQ chromatography | Clarkia breweri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
anther | reduced BAMT activity | Clarkia breweri | - |
corolla tube | reduced BAMT activity | Clarkia breweri | - |
additional information | no BAMT activity in pistil, stamen, sepal, ovary and leaf tissue | Clarkia breweri | - |
petal | in upper and lower lobes, major activity of BAMT | Clarkia breweri | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
emission of methyl benzoate begins at anthesis but at a very low level, 1.8 mg per flower for 24 hr, reaches a peak between days 5 to 8, and declines thereafter, at peak, 56.5 mg is emitted per flower in 24 hr | Clarkia breweri |
0.0004518 | - |
recombinant protein, with benzoic acid as substrate, pH 6.9, 20°C | Clarkia breweri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity detected with salicylic acid and trans-cinnamic acid and their derivatives 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, benzyl alcohol, and 2-,3- and 4-coumaric acid | Clarkia breweri | ? | - |
? | |
S-adenosyl-L-methionine + benzoate | emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity | Clarkia breweri | S-adenosyl-L-homocysteine + methyl benzoate | - |
? | |
S-adenosyl-L-methionine + benzoate | methyl benzoate is the most abundant scent compound detected in the majority of snapdragon varieties | Clarkia breweri | S-adenosyl-L-homocysteine + methyl benzoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BAMT | - |
Clarkia breweri |
S-adenosyl-L-methionine:benzoic acid carboxyl methyl transferase | - |
Clarkia breweri |
Organism | Comment | Expression |
---|---|---|
Clarkia breweri | during flower life span, levels of methyl benzoate emission, BAMT activity, BAMT gene expression, and the amounts of BAMT protein and benzoic acid are developmentally and differentially regulated | additional information |
General Information | Comment | Organism |
---|---|---|
metabolism | final enzyme in the biosynthesis of methyl benzoate | Clarkia breweri |
physiological function | attracting pollinators like bumblebees | Clarkia breweri |