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Literature summary for 2.1.1.278 extracted from
- Molecular cloning and biochemical characterization of indole-3-acetic acid methyltransferase from poplar (2007), Phytochemistry, 68, 1537-1544.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) CodonPlus cells | Populus trichocarpa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | complete inhibition at 5 mM | Populus trichocarpa |  |
| Fe2+ | nearly complete inhibition at 5 mM | Populus trichocarpa | |
| Mg2+ | about 80% residual activity at 5 mM | Populus trichocarpa | |
| Mn2+ | about 50% residual activity at 5 mM | Populus trichocarpa | |
| Zn2+ | nearly complete inhibition at 5 mM | Populus trichocarpa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0231 | - |
S-adenosyl-L-methionine | at pH 7.5 and 25°C | Populus trichocarpa | |
| 0.0304 | - |
(indol-3-yl)acetate | at pH 7.5 and 25°C | Populus trichocarpa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | mild activation at 5 mM | Populus trichocarpa | |
| K+ | about 225% activity at 5 mM | Populus trichocarpa | |
| Na+ | mild activation at 5 mM | Populus trichocarpa | |
| NH4+ | mild activation at 5 mM | Populus trichocarpa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Populus trichocarpa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + (indol-3-yl)acetate | Populus trichocarpa | highest activity | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus trichocarpa | - |
clone Nisqually-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column agarose chromatography | Populus trichocarpa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | moderate expression in young leaves, no expression in old leaves | Populus trichocarpa | - |
| root | low level of expression | Populus trichocarpa | - |
| stem | highest level of expression | Populus trichocarpa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with salicylic acid, benzoic acid, jasmonic acid, and farnesoic acid | Populus trichocarpa | ? | - |
? | |
| S-adenosyl-L-methionine + (indol-3-yl)acetate | highest activity | Populus trichocarpa | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
| S-adenosyl-L-methionine + (indol-3-yl)butyrate | 11% activity compared to (indol-3-yl)acetate | Populus trichocarpa | S-adenosyl-L-homocysteine + methyl (indol-3-yl)butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Populus trichocarpa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IAMT | - |
Populus trichocarpa |
| indole-3-acetic acid methyltransferase | - |
Populus trichocarpa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Populus trichocarpa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 40 | activity at 4°C and 40°C is about 40% of the maximal activity | Populus trichocarpa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Populus trichocarpa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 9 | at pH 6.5, the enzyme has about 34% of its maximal activity, whereas at pH 9.0, the enzyme displays about 40% of the maximal activity | Populus trichocarpa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in development of root and leaf in poplar and plays a role in wood formation | Populus trichocarpa |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.782 | - |
S-adenosyl-L-methionine | at pH 7.5 and 25°C | Populus trichocarpa | |
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