Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxymercuribenzoate | 0.00001 mM, 75% loss of activity | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
sucrose density gradient centrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + phenylethanolamine = S-adenosyl-L-homocysteine + N-methylphenylethanolamine | random binding mechanism with a kinetically preferred order of binding where S-adenosyl-L-methionine is the preferred first substrate | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal medulla | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
normetanephrine + S-adenosyl-L-methionine | - |
Bos taurus | metanephrine + S-adenosyl-L-homocysteine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
presence of 2.5 mM normetanephrine has no effect on the heat denaturation, 1 mM S-adenosyl-L-methionine decreases the rate of inactivation | Bos taurus |
39 | - |
stable for at least 50 min | Bos taurus |
45 | - |
activity is rapidly destroyed | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
Tris or borate buffer | Bos taurus |