Literature summary for 2.1.1.28 extracted from

Qin, N.; Peitzsch, M.; Menschikowski, M.; Siegert, G.; Pacak, K.; Eisenhofer, G.
Double stable isotope ultra performance liquid chromatographic-tandem mass spectrometric quantification of tissue content and activity of phenylethanolamine N-methyltransferase, the crucial enzyme responsible for synthesis of epinephrine (2013), Anal. Bioanal. Chem., 405, 1713-1719.

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Application

Application	Comment	Organism
analysis	method to measure tissue contents and activity of phenylethanolamine N-methyltransferase, utilizing double stable isotope ultra performance liquid chromatography-tandem mass spectrometry. The method is based on measurement of deuterium-labeled epinephrine produced from reaction of norepinephrine with deuterium-labeled S-adenosyl-Lmethionine as the methyl donor. In addition to enzyme activity the method allows for determination of tissue contents of PNMT using human recombinant enzyme for calibration. The calibration curve for epinephrine is linear over the range of 0.1 to 5000 pM, with 0.5 pM epinephrine representing the lower limit of quantification	Homo sapiens
medicine	strong relationship between PNMT gene expression and tissue epinephrine levels in human pheochromocytomas	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	tissue from pheochromocytoma tumor specimens	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
PNMT	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	strong relationship between PNMT gene expression and tissue epinephrine levels in human pheochromocytomas	additional information

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Release 2023.1 (January 2023)