Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli C41(DE3) | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
crystallized in the presence of S-adenosylmethionine at 223°C using ammonium sulfate as the precipitant. X-ray diffraction data are collected to 2.5 A resolution from a native crystal. The crystal is orthorhombic, belonging to the space group I222 (or I2(1)2(1)2(1)), with unit-cell parameters of a = 104.24, b = 118.73, and c = 146.62 A. Two (or three) monomers of recombinant HemK are likely to be present in the crystallographic asymmetric unit | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine | Thermotoga maritima | - |
S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine | - |
? | |
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine | Thermotoga maritima DSM 3109 | - |
S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYV8 | - |
- |
Thermotoga maritima DSM 3109 | Q9WYV8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine | - |
Thermotoga maritima | S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine | - |
? | |
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine | - |
Thermotoga maritima DSM 3109 | S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HemK | - |
Thermotoga maritima |
N5-glutamine MTase | - |
Thermotoga maritima |
N5-glutamine S-adenosyl-L-methionine dependent methyltransferase | - |
Thermotoga maritima |
PrmC | - |
Thermotoga maritima |
TM0488 | locus name | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme modifies the glutamine residue in the universally conserved glycylglycylglutamine (GGQ) motif of peptide chain release factor, resulting in almost complete loss of release activity | Thermotoga maritima |