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Literature summary for 2.1.1.297 extracted from

  • Yoon, H.J.; Kang, K.Y.; Ahn, H.J.; Shim, S.M.; Ha, J.Y.; Lee, S.K.; Mikami, B.; Suh, S.W.
    X-ray crystallographic studies of HemK from Thermotoga maritima, an N5-glutamine methyltransferase (2003), Mol. Cells, 16, 266-269.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli C41(DE3) Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized in the presence of S-adenosylmethionine at 223°C using ammonium sulfate as the precipitant. X-ray diffraction data are collected to 2.5 A resolution from a native crystal. The crystal is orthorhombic, belonging to the space group I222 (or I2(1)2(1)2(1)), with unit-cell parameters of a = 104.24, b = 118.73, and c = 146.62 A. Two (or three) monomers of recombinant HemK are likely to be present in the crystallographic asymmetric unit Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine Thermotoga maritima
-
S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
-
?
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine Thermotoga maritima DSM 3109
-
S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYV8
-
-
Thermotoga maritima DSM 3109 Q9WYV8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine
-
Thermotoga maritima S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
-
?
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine
-
Thermotoga maritima DSM 3109 S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
-
?

Synonyms

Synonyms Comment Organism
HemK
-
Thermotoga maritima
N5-glutamine MTase
-
Thermotoga maritima
N5-glutamine S-adenosyl-L-methionine dependent methyltransferase
-
Thermotoga maritima
PrmC
-
Thermotoga maritima
TM0488 locus name Thermotoga maritima

General Information

General Information Comment Organism
physiological function the enzyme modifies the glutamine residue in the universally conserved glycylglycylglutamine (GGQ) motif of peptide chain release factor, resulting in almost complete loss of release activity Thermotoga maritima