Cloned (Comment) | Organism |
---|---|
plasmid pSH25 carrying N terminal 6xHis-Smt3tagged Bmt6, is constructed using pPK591 by gap repair. For overexpression, BL21 (DE3) cells are transformed with isolated plasmids and grown in LB medium containing 100 mg/ml ampicillin at 37°C to an OD600 of 0.6 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | Bmt6 is localized predominantly in the cytoplasm | Saccharomyces cerevisiae | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil2843 in 25S rRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N3-methyluracil2843 in 25S rRNA | - |
? | |
S-adenosyl-L-methionine + uracil2843 in 25S rRNA | Saccharomyces cerevisiae ATCC 204508 | - |
S-adenosyl-L-homocysteine + N3-methyluracil2843 in 25S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12291 | - |
- |
Saccharomyces cerevisiae ATCC 204508 | Q12291 | - |
- |
Purification (Comment) | Organism |
---|---|
His-tagged enzyme is purified by Nickel-NTA metal affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil2843 in 25S rRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N3-methyluracil2843 in 25S rRNA | - |
? | |
S-adenosyl-L-methionine + uracil2843 in 25S rRNA | - |
Saccharomyces cerevisiae ATCC 204508 | S-adenosyl-L-homocysteine + N3-methyluracil2843 in 25S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BMT6 | - |
Saccharomyces cerevisiae |
YLR063W | locus name | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of bmt6 does not impair the growth of the cells at 30°C, 37°C and 19°C on YPD media respectively. The loss of bmt6 does not exhibit any hypersensitivity to paromomycin and anisomycin. The absence of the N3-methyluracil2843 modification does not disturb ribosomal stoichiometry and affect translation | Saccharomyces cerevisiae |