Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as N-terminal polyhistidine-tagged protein | Streptomyces lavendulae |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystal structures of the mitomycin-7-O-methyltransferaseS-adenosylhomocysteine binary complex and mitomycin-7-O-methyltransferaseS-adenosyl-L-homocysteinemitomycin A ternary complex at resolutions of 1.9 and 2.3 A, respectively | Streptomyces lavendulae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces lavendulae | the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin | ? | - |
? | |
additional information | Streptomyces lavendulae NRRL 2564 | the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae | Q9X5T6 | - |
- |
Streptomyces lavendulae NRRL 2564 | Q9X5T6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces lavendulae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + 6-demethylmitomycin A = S-adenosyl-L-homocysteine + mitomycin A | (1) | Streptomyces lavendulae | |
S-adenosyl-L-methionine + 6-demethylmitomycin B = S-adenosyl-L-homocysteine + mitomycin B | (2) | Streptomyces lavendulae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin | Streptomyces lavendulae | ? | - |
? | |
additional information | the enzyme catalyzes the 7-O-methylation of both C9beta- and C9alpha-configured 7-hydroxymitomycins | Streptomyces lavendulae | ? | - |
? | |
additional information | the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin | Streptomyces lavendulae NRRL 2564 | ? | - |
? | |
additional information | the enzyme catalyzes the 7-O-methylation of both C9beta- and C9alpha-configured 7-hydroxymitomycins | Streptomyces lavendulae NRRL 2564 | ? | - |
? | |
S-adenosyl-L-methionine + 6-demethylmitomycin A | - |
Streptomyces lavendulae | S-adenosyl-L-homocysteine + mitomycin A | - |
? | |
S-adenosyl-L-methionine + 6-demethylmitomycin A | - |
Streptomyces lavendulae NRRL 2564 | S-adenosyl-L-homocysteine + mitomycin A | - |
? | |
S-adenosyl-L-methionine + 6-demethylmitomycin B | - |
Streptomyces lavendulae | S-adenosyl-L-homocysteine + mitomycin B | - |
? | |
S-adenosyl-L-methionine + 6-demethylmitomycin B | - |
Streptomyces lavendulae NRRL 2564 | S-adenosyl-L-homocysteine + mitomycin B | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Streptomyces lavendulae |
Synonyms | Comment | Organism |
---|---|---|
MmcR | - |
Streptomyces lavendulae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin | Streptomyces lavendulae |