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Literature summary for 2.1.1.318 extracted from

  • Sengupta, S.; Banerjee, S.; Lahiri, S.; Dutta, T.; Dhar, T.K.; Ghosh, A.K.
    Purification, characterization, sequencing and molecular cloning of a novel cysteine methyltransferase that regulates trehalose-6-phosphate synthase from Saccharomyces cerevisiae (2014), Biochim. Biophys. Acta, 1840, 1861-1871.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 2.5 mM, 1.3fold activation Saccharomyces cerevisiae
EGTA 2.5 mM, 1.3fold activation Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Citric acid 2.5 mM, 25% inhibition Saccharomyces cerevisiae
S-adenosyl-L-homocysteine competitive Saccharomyces cerevisiae
Sodium azide 2.5 mM; 40% inhibition Saccharomyces cerevisiae
sulfosalicylic acid 2.5 mM, 35% inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00495
-
S-adenosyl-L-methionine 37°C, pH not specified in the publication, native and cloned enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
ZnCl2 5 mM, enhances activity 1.4fold Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
non-denaturing PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + [trehalose-6-phosphate synthase]-L-cysteine Saccharomyces cerevisiae the enzyme performs S-methylation at cysteine residue regulating trehalose-6-phosphate synthase activity by 50%, which results in an increase of the intercellular stress sugar, trehalose. It contributes to stationary phase survival of Saccharomyces cerevisiae by elevating the levels of trehalose in the cell as it enters the stationary phase S-adenosyl-L-homocysteine + [trehalose-6-phosphate synthase]-S-methyl-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae I6WHP7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + [albumin]-L-cysteine in vitro, the activity of the purified enzyme predominantly corresponds with the cysteine content of the substrates and is not specific for trehalose-6-phosphate synthase Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [albumin]-S-methyl-L-cysteine
-
?
S-adenosyl-L-methionine + [chymotrypsin]-L-cysteine
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [chymotrypsin]-S-methyl-L-cysteine
-
?
S-adenosyl-L-methionine + [lipase]-L-cysteine
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [lipase]-S-methyl-L-cysteine
-
?
S-adenosyl-L-methionine + [ovalbumin]-L-cysteine
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [ovalbumin]-S-methyl-L-cysteine
-
?
S-adenosyl-L-methionine + [peroxidase]-L-cysteine
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [peroxidase]-S-methyl-L-cysteine
-
?
S-adenosyl-L-methionine + [ribonuclease A]-L-cysteine
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [ribonuclease A]-S-methyl-L-cysteine
-
?
S-adenosyl-L-methionine + [trehalose-6-phosphate synthase]-L-cysteine
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [trehalose-6-phosphate synthase]-S-methyl-L-cysteine
-
?
S-adenosyl-L-methionine + [trehalose-6-phosphate synthase]-L-cysteine the enzyme performs S-methylation at cysteine residue regulating trehalose-6-phosphate synthase activity by 50%, which results in an increase of the intercellular stress sugar, trehalose. It contributes to stationary phase survival of Saccharomyces cerevisiae by elevating the levels of trehalose in the cell as it enters the stationary phase Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [trehalose-6-phosphate synthase]-S-methyl-L-cysteine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 14000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4
-
S-adenosyl-L-methionine 37°C, pH not specified in the publication, native enzyme Saccharomyces cerevisiae
7
-
S-adenosyl-L-methionine 37°C, pH not specified in the publication, cloned enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0109
-
S-adenosyl-L-homocysteine 37°C, pH not specified in the publication Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae calculated from sequence
-
6.82

General Information

General Information Comment Organism
malfunction trehalose concentration is 0.93 mM/mg wet weight in the wild type strain as compared to 0.51 mMl/mg wetweight in the knock out mutant strains Saccharomyces cerevisiae
physiological function methylation of trehalose-6-phosphate synthase results in enhanced activity and subsequently increased trehalose production in the cell as it enters the stationary phase Saccharomyces cerevisiae