Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2.5 mM, 1.3fold activation | Saccharomyces cerevisiae | |
EGTA | 2.5 mM, 1.3fold activation | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Citric acid | 2.5 mM, 25% inhibition | Saccharomyces cerevisiae | |
S-adenosyl-L-homocysteine | competitive | Saccharomyces cerevisiae | |
Sodium azide | 2.5 mM; 40% inhibition | Saccharomyces cerevisiae | |
sulfosalicylic acid | 2.5 mM, 35% inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00495 | - |
S-adenosyl-L-methionine | 37°C, pH not specified in the publication, native and cloned enzyme | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
ZnCl2 | 5 mM, enhances activity 1.4fold | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
non-denaturing PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [trehalose-6-phosphate synthase]-L-cysteine | Saccharomyces cerevisiae | the enzyme performs S-methylation at cysteine residue regulating trehalose-6-phosphate synthase activity by 50%, which results in an increase of the intercellular stress sugar, trehalose. It contributes to stationary phase survival of Saccharomyces cerevisiae by elevating the levels of trehalose in the cell as it enters the stationary phase | S-adenosyl-L-homocysteine + [trehalose-6-phosphate synthase]-S-methyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | I6WHP7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [albumin]-L-cysteine | in vitro, the activity of the purified enzyme predominantly corresponds with the cysteine content of the substrates and is not specific for trehalose-6-phosphate synthase | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [albumin]-S-methyl-L-cysteine | - |
? | |
S-adenosyl-L-methionine + [chymotrypsin]-L-cysteine | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [chymotrypsin]-S-methyl-L-cysteine | - |
? | |
S-adenosyl-L-methionine + [lipase]-L-cysteine | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [lipase]-S-methyl-L-cysteine | - |
? | |
S-adenosyl-L-methionine + [ovalbumin]-L-cysteine | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [ovalbumin]-S-methyl-L-cysteine | - |
? | |
S-adenosyl-L-methionine + [peroxidase]-L-cysteine | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [peroxidase]-S-methyl-L-cysteine | - |
? | |
S-adenosyl-L-methionine + [ribonuclease A]-L-cysteine | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [ribonuclease A]-S-methyl-L-cysteine | - |
? | |
S-adenosyl-L-methionine + [trehalose-6-phosphate synthase]-L-cysteine | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [trehalose-6-phosphate synthase]-S-methyl-L-cysteine | - |
? | |
S-adenosyl-L-methionine + [trehalose-6-phosphate synthase]-L-cysteine | the enzyme performs S-methylation at cysteine residue regulating trehalose-6-phosphate synthase activity by 50%, which results in an increase of the intercellular stress sugar, trehalose. It contributes to stationary phase survival of Saccharomyces cerevisiae by elevating the levels of trehalose in the cell as it enters the stationary phase | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [trehalose-6-phosphate synthase]-S-methyl-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 14000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
S-adenosyl-L-methionine | 37°C, pH not specified in the publication, native enzyme | Saccharomyces cerevisiae | |
7 | - |
S-adenosyl-L-methionine | 37°C, pH not specified in the publication, cloned enzyme | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
S-adenosyl-L-homocysteine | 37°C, pH not specified in the publication | Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | calculated from sequence | - |
6.82 |
General Information | Comment | Organism |
---|---|---|
malfunction | trehalose concentration is 0.93 mM/mg wet weight in the wild type strain as compared to 0.51 mMl/mg wetweight in the knock out mutant strains | Saccharomyces cerevisiae |
physiological function | methylation of trehalose-6-phosphate synthase results in enhanced activity and subsequently increased trehalose production in the cell as it enters the stationary phase | Saccharomyces cerevisiae |