Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species | Escherichia coli |
300000 | - |
gel filtration, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
2 isoenzymes | - |
Escherichia coli MRE 600 | - |
2 isoenzymes | - |
Purification (Comment) | Organism |
---|---|
1000fold purification by affinity chromatography on Escherichia coli tRNA Sepharose | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.95 | - |
tRNAfMet from Bacillus subtilis | Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, frozen in liquid nitrogen, at least several months, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | methyl group transfer at the polynucleotide level of tRNA, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species | Escherichia coli | additional information | - |
? | |
additional information | methyl group transfer at the polynucleotide level of tRNA, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species | Escherichia coli MRE 600 | additional information | - |
? | |
S-adenosyl-L-methionine + guanine46 in tRNA | methyl group acceptors: methyl-deficient bulk tRNA from E. coli K12met-rel-, tRNAfMet from Bacillus subtilis, formation of 0.8 mol N7-methylguanine residues per mol Bacillus subtilis tRNA, enzyme recognizes and methylates specifically the extra arm unpaired methyl-deficient guanylate residue | Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine46 in tRNA | methyl group acceptors: methyl-deficient bulk tRNA from E. coli K12met-rel-, tRNAfMet from Bacillus subtilis, formation of 0.8 mol N7-methylguanine residues per mol Bacillus subtilis tRNA, enzyme recognizes and methylates specifically the extra arm unpaired methyl-deficient guanylate residue | Escherichia coli MRE 600 | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | serves usually as coenzyme | Escherichia coli |