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Literature summary for 2.1.1.33 extracted from

  • Aschhoff, H.J.; Elten, H.; Arnold, H.H.; Mahal, G.; Kersten, W.; Kersten, H.
    7-Methylguanine specific tRNA-methyltransferase from Escherichia coli (1976), Nucleic Acids Res., 3, 3109-3122.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Escherichia coli
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species Escherichia coli
300000
-
gel filtration, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
2 isoenzymes
-
Escherichia coli MRE 600
-
2 isoenzymes
-

Purification (Commentary)

Purification (Comment) Organism
1000fold purification by affinity chromatography on Escherichia coli tRNA Sepharose Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.95
-
tRNAfMet from Bacillus subtilis Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, frozen in liquid nitrogen, at least several months, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information methyl group transfer at the polynucleotide level of tRNA, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species Escherichia coli additional information
-
?
additional information methyl group transfer at the polynucleotide level of tRNA, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species Escherichia coli MRE 600 additional information
-
?
S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptors: methyl-deficient bulk tRNA from E. coli K12met-rel-, tRNAfMet from Bacillus subtilis, formation of 0.8 mol N7-methylguanine residues per mol Bacillus subtilis tRNA, enzyme recognizes and methylates specifically the extra arm unpaired methyl-deficient guanylate residue Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
?
S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptors: methyl-deficient bulk tRNA from E. coli K12met-rel-, tRNAfMet from Bacillus subtilis, formation of 0.8 mol N7-methylguanine residues per mol Bacillus subtilis tRNA, enzyme recognizes and methylates specifically the extra arm unpaired methyl-deficient guanylate residue Escherichia coli MRE 600 S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine serves usually as coenzyme Escherichia coli