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Literature summary for 2.1.1.33 extracted from

  • Liu, Q.; Gao, Y.; Yang, W.; Zhou, H.; Gao, Y.; Zhang, X.; Teng, M.; Niu, L.
    Crystallization and preliminary crystallographic analysis of tRNA (m7G46) methyltransferase from Escherichia coli (2008), Acta Crystallogr. Sect. F, F64, 743-745.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
a truncated protein lacking the first 32 residues of the N-terminus but with an additional His6 tag at the C-terminus is crystallized by hanging-drop vapour-diffusion method using 15% (w/v) PEG 3350 and 150 mM MgSO4, at 10°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27307
-
x * 27307, calculated from sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine46 in tRNA Escherichia coli
-
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A8I5 strain K12
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine46 in tRNA
-
Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
?

Subunits

Subunits Comment Organism
? x * 27307, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
transfer RNA (m7G46) methyltransferase
-
Escherichia coli
TrmB TrmB belongs to the Rossmann-fold methyltransferase family Escherichia coli
tRNA (m7G46) methyltransferase
-
Escherichia coli