Literature summary for 2.1.1.34 extracted from

Pleshe, E.; Truesdell, J.; Batey, R.T.
Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus (2005), Acta Crystallogr. Sect. F, 61, 722-728.

Search for more literature for 2.1.1.34

Application

Application	Comment	Organism
analysis	reveals a fold typical of members of the SpoU clan of proteins, a subfamily of the alpha/beta-knot superfamily, with alpha-helical extensions at the N- and C-termini that are likely to be involved in tRNA binding	Aquifex aeolicus

Cloned(Commentary)

Cloned (Comment)	Organism
into vector pThioHisB and transformed into Escherichia coli strain DH5alpha, overexpressed in Escherichia coli Rosetta(DE3)/pLysS cells	Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
by the hanging-drop method, at 1.85 A resolution, diffraction-quality crystals in the pH range 4.04.5, enzyme can not be crystallized with S-adenosyl-L-methionine or an analog in the active site	Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	gel filtration	Aquifex aeolicus

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	O67577	-	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration, more than 99% pure	Aquifex aeolicus

Subunits

Subunits	Comment	Organism
homodimer	gel filtration	Aquifex aeolicus

Synonyms

Synonyms	Comment	Organism
TrmH	-	Aquifex aeolicus

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Release 2023.1 (January 2023)