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Literature summary for 2.1.1.35 extracted from

  • Björk, G.R.; Svensson, I.
    Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae (1969), Eur. J. Biochem., 9, 207-215.
    View publication on PubMed
Search for more literature for 2.1.1.35

General Stability

General Stability Organism
freezing without antifreeze agents inactivates, 30% ethylene glycol at -20°C stabilizes, dialysis inactivates, best pH used for dialysis is 6.5 Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ inhibits at low concentrations Saccharomyces cerevisiae
Mn2+ inhibits at low concentrations Saccharomyces cerevisiae
NH4+ at 0.1 M, 50% inhibition Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no divalent cations required, not activated by Mg2+ and Mn2+ Saccharomyces cerevisiae

Organic Solvent Stability

Organic Solvent Comment Organism
Ethylene glycol 30%, stabilizes at -20°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae D84
-
-
-

Purification (Commentary)

Purification (Comment) Organism
may be 3 isoenzymes Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Saccharomyces cerevisiae D84 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae