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Literature summary for 2.1.1.35 extracted from
- Isolation and characterization of m5U-methyltransferase from Escherichia coli (1980), J. Biol. Chem., 255, 8296-8302.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermidine | slight activation at 20 mM in presence of 50 mM ammonium acetate | Escherichia coli |  |
General Stability
| General Stability | Organism |
|---|---|
| freeze-thawing inactivates | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-homocysteine | at 0.002 mM 50% inhibition, E. coli MRE 600 | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
uracil54 in wheat germ tRNA1-Gly | - |
Escherichia coli | |
| 0.0125 | - |
S-adenosyl-L-methionine | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| ammonium acetate | 30% activation at 50 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
gel filtration | Escherichia coli |
| 42000 | - |
1 * 42000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli | tRNA modifying enzyme | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli MRE 600 | tRNA modifying enzyme | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli SN01 | tRNA modifying enzyme | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli | - |
MRE 600 | - |
| Escherichia coli MRE 600 | - |
MRE 600 | - |
| Escherichia coli SN01 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| polyethyleneimine precipitation, phosphocellulose and affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0104 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, at least 6 months, stable | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA | Escherichia coli | additional information | - |
? | |
| additional information | poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA | Escherichia coli MRE 600 | additional information | - |
? | |
| additional information | poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA | Escherichia coli SN01 | additional information | - |
? | |
| S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly | - |
? | |
| S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly | - |
Escherichia coli MRE 600 | S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly | - |
? | |
| S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly | - |
Escherichia coli SN01 | S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly | - |
? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | tRNAs from wheat germ are the best substrates | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3 end | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | tRNA modifying enzyme | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | tRNAs from wheat germ are the best substrates | Escherichia coli MRE 600 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3 end | Escherichia coli MRE 600 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | tRNA modifying enzyme | Escherichia coli MRE 600 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | tRNAs from wheat germ are the best substrates | Escherichia coli SN01 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3 end | Escherichia coli SN01 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
| S-adenosyl-L-methionine + uridine54 in tRNA | tRNA modifying enzyme | Escherichia coli SN01 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.8, Escherichia coli MRE 600 | Escherichia coli |
| 8.4 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.8 | 25% inhibition of enzyme activity at pH 7.8 and 8.8 | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Escherichia coli | |
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