Literature summary for 2.1.1.35 extracted from

Gu, X.; Matsuda, A.; Ivanetich, K.M.; Santi, D.V.
Interaction of tRNA (uracil-5-)-methyltransferase with NO2Ura-tRNA (1996), Nucleic Acids Res., 24, 1059-1064.

Search for more literature for 2.1.1.35

Inhibitors

Inhibitors	Comment	Organism	Structure
5-Fluorouracil substituted tRNA	-	Escherichia coli
NO2Ura-tRNA	potent mechanism-based inhibitor, competitive with respect to tRNA, can be reversed by dilution into a large excess of tRNA substrate, forms a reversible covalent complex in which Cys-324 of RUMT is linked to the 6-position of NO2-Ura 54 in NO2-Ura-tRNA, mechanism	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00037	-	uracil54 in yeast tRNA-Phe	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA	catalytic mechanism	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe	-	Escherichia coli	S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe	-	?
S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe	-	Escherichia coli	S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe	-	r
S-adenosyl-L-methionine + uridine54 in tRNA	methyl group acceptor: unmodified yeast tRNAPhe	Escherichia coli	S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
15	-	assay at	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0317	-	uracil54 in yeast tRNA1-Phe	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.001	-	NO2Ura-tRNA	-	Escherichia coli

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Release 2023.1 (January 2023)