Cloned (Comment) | Organism |
---|---|
cloning and sequencing of the TRM2 gene encoding enzyme, gene is previously identified as RNC1/NUD1 gene, but does not encode the yNucR endo-exonuclease, TRM2 gene is not essential for cell viability and contains alternative translational start sites, glutathione S-transferase tagged recombinant, Trm2p, is expressed in Escherichia coli | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | the m5U modification is found in unspliced tRNAs, suggesting that enzyme action is within the nucleus | Saccharomyces cerevisiae | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | enzyme is responsible for the formation of 5-methyluridine at position 54 in tRNA | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
several strains | - |
Purification (Comment) | Organism |
---|---|
purification of glutathione S-transferase tagged recombinant, Trm2p, expressed in Escherichia coli BL21 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | enzyme is responsible for the formation of 5-methyluridine at position 54 in tRNA | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: yeast tRNA | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | nearly all tRNAs contain m5U54, initiators excluded | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39 | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | nearly all tRNAs contain m5U54, initiators excluded | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae |