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Literature summary for 2.1.1.37 extracted from

  • Pfeifer, G.P.; Grunwald, S.; Boehm, T.L.J.; Drahovsky, D.
    Isolation and characterization of DNA cytosine 5-methyltransferase from human placenta (1983), Biochim. Biophys. Acta, 740, 323-330.
    View publication on PubMed
Search for more literature for 2.1.1.37

Inhibitors

Inhibitors Comment Organism Structure
EDTA above 10 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 S-adenosyl-L-methionine
-
 Homo sapiens
0.0077
-
 native Micrococcus lysodeikticus DNA containing cytosine
-
 Homo sapiens
0.015
-
 P815 DNA containing cytosine
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 glycerol gradient centrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta soft tissue of Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000166
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 30% glycerol, activity decays at a rate of about 15% in 2 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
 Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + DNA the enzyme methylates DNA from various sources in native and heat-denatured forms. The synthetic copolymer poly(dG-dC)*poly(dG-dC) is methylated in B-conformation and in Z-conformation to about the same extent, determination of maintenance DNA methylase activity with P815 DNA and determination of de novo DNA methylase activity with 5-methylcytosine-free procaryotic DNA Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + native Micrococcus lysodeikticus DNA containing cytosine
-
 Homo sapiens S-adenosyl-L-homocysteine + native Micrococcus lysodeikticus DNA containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + P815 DNA containing cytosine
-
 Homo sapiens S-adenosyl-L-homocysteine + P815 DNA containing 5-methylcytosine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Homo sapiens