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Literature summary for 2.1.1.37 extracted from

  • Dubey, A.K.; Mollet, B.; Roberts, R.J.
    Purification and characterization of the MspI DNA methyltransferase cloned and overexpressed in E. coli (1992), Nucleic Acids Res., 20, 1579-1585.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000 54000 gel filtration Moraxella sp.
49000
-
1 * 49000, at low concentrations, less than 0.4 mg/ml, the enzyme is a monomer in solution but at higher concentrations, greater than 3.0 mg/ml it exists predominantly as a dimer, SDS-PAGE Moraxella sp.

Organism

Organism UniProt Comment Textmining
Moraxella sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
Moraxella sp. S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?
S-adenosyl-L-methionine + DNA the enzyme recognizes the sequence 5'-CCGG-3' and methylates the outer cytosine residue Moraxella sp. S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 49000, at low concentrations, less than 0.4 mg/ml, the enzyme is a monomer in solution but at higher concentrations, greater than 3.0 mg/ml it exists predominantly as a dimer, SDS-PAGE Moraxella sp.

Synonyms

Synonyms Comment Organism
M.MspI
-
Moraxella sp.
MspI DNA methyltransferase
-
Moraxella sp.