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Literature summary for 2.1.1.37 extracted from

  • Timar, E.; Venetianer, P.; Kiss, A.
    In vivo DNA protection by relaxed-specificity SinI DNA methyltransferase variants (2008), J. Bacteriol., 190, 8003-8008.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Salmonella enterica

Protein Variants

Protein Variants Comment Organism
A34V/K44Q/M66T/L214S/Y229H isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites Salmonella enterica
L214S/Y229H isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites Salmonella enterica
N172S isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites, the mutant enzyme displays enhanced protection of the cell DNA against the Sau96I endonuclease. The protection is not sufficient to support long-term survival in the presence of the inducer, which is consistent with incomplete methylation of GG(G/C)CC sites in plasmid DNA purified from the mutant Salmonella enterica
V173L isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites, the mutant enzyme displays enhanced protection of the cell DNA against the Sau96I endonuclease. The protection is not sufficient to support long-term survival in the presence of the inducer, which is consistent with incomplete methylation of GG(G/C)CC sites in plasmid DNA purified from the mutant Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + S-adenosyl-L-methionine Salmonella enterica SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
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 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
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 serovar Infantis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + S-adenosyl-L-methionine SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine Salmonella enterica DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?
DNA + S-adenosyl-L-methionine SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine, molecular mechanism of substrate recognition, overview Salmonella enterica DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
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 ?

Synonyms

Synonyms Comment Organism
SinI DNA methyltransferase
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 Salmonella enterica

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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 Salmonella enterica