Application | Comment | Organism |
---|---|---|
agriculture | construction of transgenic plants with modified sterol profile via recombinant sterol 24-C-methyltransferase shall protect crops against damage by insect infestation | Saccharomyces cerevisiae |
agriculture | construction of transgenic plants with modified sterol profile via recombinant sterol 24-C-methyltransferase shall protect crops against damage by insect infestation | Candida albicans |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(24R,S),25-epiminolanosterol | - |
Saccharomyces cerevisiae | |
(3beta,24,R,S)-24-methyl-24-thioniacholest-5-enol | - |
Candida albicans | |
24(S)-24-methyl-25-thiacholest-5-enol iodide | - |
Saccharomyces cerevisiae | |
25-Aza-24,25-dihydrozymosterol | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Saccharomyces cerevisiae | - |
- |
microsome | - |
Candida albicans | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
zymosterol + S-adenosyl-L-methionine | Saccharomyces cerevisiae | role in biosynthesis of plant and fungi sterols | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | Candida albicans | role in biosynthesis of plant and fungi sterols | fecosterol + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | mechanism | Saccharomyces cerevisiae | |
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | mechanism | Candida albicans | |
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | active site model, termed steric-electric plug model, consists of a non-covalent mechanism involving the intermediacy of a 24beta-methyl (or ethyl) sterol bound to the ternary complex | Saccharomyces cerevisiae | |
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | active site model, termed steric-electric plug model, consists of a non-covalent mechanism involving the intermediacy of a 24beta-methyl (or ethyl) sterol bound to the ternary complex | Candida albicans | |
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | substrate binding, amino acid sequence | Saccharomyces cerevisiae | |
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | substrate binding, amino acid sequence | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cycloartenol + S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
cycloartenol + S-adenosyl-L-methionine | - |
Candida albicans | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
lanosterol + S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine | - |
? | |
lanosterol + S-adenosyl-L-methionine | - |
Candida albicans | 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Candida albicans | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | role in biosynthesis of plant and fungi sterols | Saccharomyces cerevisiae | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | role in biosynthesis of plant and fungi sterols | Candida albicans | fecosterol + S-adenosyl-L-homocysteine | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000003 | - |
25-Aza-24,25-dihydrozymosterol | - |
Saccharomyces cerevisiae | |
0.000016 | - |
(3beta,24,R,S)-24-methyl-24-thioniacholest-5-enol | - |
Candida albicans |