Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Glycine max | |
EDTA | - |
Petroselinum crispum | |
S-adenosyl-L-homocysteine | inhibits the methylation of luteolin by S-adenosylmethionine | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
S-adenosyl-L-methionine | with luteolin as substrate | Glycine max | |
0.016 | - |
5,7,3',4'-tetrahydroxyflavone | - |
Glycine max | |
0.028 | - |
5,7,3'-trihydroxyflavone 7-O-glucoside | - |
Glycine max | |
0.035 | - |
6,7-dihydroxy-3'-methoxyisoflavone | - |
Glycine max | |
0.075 | - |
5,7,3',4'-tetrahydroxyflavanone | - |
Glycine max | |
0.227 | - |
5-hydroxy-3-methoxy-4-hydroxycinnamic acid | - |
Glycine max | |
0.435 | - |
dihydroquercetin | - |
Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Glycine max | |
Mg2+ | required for activity | Petroselinum crispum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean, L. var. Mandarin | - |
Petroselinum crispum | - |
parsley | - |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0029 | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
-20°C, stored in 10% glycerol for 2 months, no singnificant loss in enzyme activity | Glycine max |
4°C, highly unstable, stored with Triton X-100, bovine serum albumin, ethylene glycol, dimethyl sulfoxide, 2-mercaptoethanol, dithiothreitol or ethylene glycol monoethylether, loses over 90% of its activity within 94 h | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Petroselinum crispum | ? | - |
? | |
additional information | methylation proceedes only in the meta-position, enzyme is unable to catalyze the methylation of p-coumaric acid, m-coumaric acid, ferulic acid, isoferulic acid, sinapic acid, apigenin and naringenin | Glycine max | ? | - |
? | |
additional information | isoflavones biochanin A and daidzein do not serve as substrates | Glycine max | ? | - |
? | |
S-adenosyl-L-homocysteine + 6,7-dihydroxy-3'-methoxyisoflavone | - |
Glycine max | ? | - |
? | |
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxy flavone | quercetin | Glycine max | S-adenosyl-L-homocysteine + isorhamnetin | - |
? | |
S-adenosyl-L-methionine + 4,5-dihydroxy-3-methoxycinnamate | 5-hydroxy ferulic acid | Glycine max | S-adenosyl-L-homocysteine + 4-hydroxy-3,5-dimethoxycinnamate | - |
? | |
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavanone | eriodictyol | Glycine max | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavanone | - |
? | |
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavanone | eriodictyol | Petroselinum crispum | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavanone | - |
? | |
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone | luteolin | Glycine max | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone | - |
? | |
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone | luteolin | Petroselinum crispum | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone | - |
? | |
S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside | - |
Glycine max | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside | chrysoeriol-7-O-glucoside | ? | |
S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside | - |
Petroselinum crispum | S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside | chrysoeriol-7-O-glucoside | ? | |
S-adenosyl-L-methionine + dihydro-3,3',4',5,7-pentahydroxy flavone | dihydroquercetin | Glycine max | S-adenosyl-L-homocysteine + dihydroisorhamnetin | - |
? | |
S-adenosyl-L-methionine + quercetin 3-O-glucoside | poor substrate | Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + rutin | poor substrate | Glycine max | S-adenosyl-L-homocysteine + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | 8.9 | methylation of quercetin | Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Glycine max |