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Literature summary for 2.1.1.42 extracted from

  • Muzac, I.; Wang, J.; Anzellotti, D.; Zhang, H.; Ibrahim, R.K.
    Functional expression of an Arabidopsis cDNA clone encoding a flavonol 3'-O-methyltransferase and characterization of the gene product (2000), Arch. Biochem. Biophys., 375, 385-388.
    View publication on PubMed
Search for more literature for 2.1.1.42

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli BL21 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 5 mM, complete inhibition Arabidopsis thaliana
NiSO4 5 mM, complete inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00176
-
 3,3',4',5,7-pentahydroxyflavone i.e. quercetin, 30°C, pH not specified in the publication Arabidopsis thaliana
0.00338
-
 3,3',4',5,5',7-hexahydroxyflavone i.e. mycricetin, 30°C, pH not specified in the publication Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
additional information does not require Mg2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 recombinant fusion protein containing a 3 kDa His tail, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone Arabidopsis thaliana i.e. quercetin. Preferred substrate S-adenosyl-L-homocysteine + 3,4',5,7-tetrahydroxy-3'-methoxyflavone i.e. isorhamnetin ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FK25
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information only flavonols with vicinal B-ring hydroxyl groups, such as quercetin and myricetin are good methyl acceptor Arabidopsis thaliana ?
-
 ?
S-adenosyl-L-methionine + 3,3',4',5,5',7-hexahydroxyflavone i.e. mycricetin. 70% of the activity with quercetin Arabidopsis thaliana S-adenosyl-L-homocysteine + 3,4',5,5',7-pentahydroxy-3'-methoxyflavone
-
 ?
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone i.e. quercetin. Preferred substrate Arabidopsis thaliana S-adenosyl-L-homocysteine + 3,4',5,7-tetrahydroxy-3'-methoxyflavone i.e. isorhamnetin ?
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. luteolin. 15% of the activity with quercetin Arabidopsis thaliana S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone
-
 ?

Subunits

Subunits Comment Organism
? 1 * 45000, recombinant fusion protein containing a 3 kDa His tail, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
caffeic acid/5-hydroxyferulic acid O-methyltransferase
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
 3,3',4',5,5',7-hexahydroxyflavone i.e. mycricetin, 30°C, pH not specified in the publication Arabidopsis thaliana
16
-
 3,3',4',5,7-pentahydroxyflavone i.e. quercetin, 30°C, pH not specified in the publication Arabidopsis thaliana