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Literature summary for 2.1.1.42 extracted from

  • Kim, B.G.; Lee, H.J.; Park, Y.; Lim, Y.; Ahn, J.H.
    Characterization of an O-methyltransferase from soybean (2006), Plant Physiol. Biochem., 44, 236-241.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
SOMT-9, DNA and amino acid sequence determination, expression of GST-tagged SOMT-9 in Escherichia coli Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
x * 64000, recombinant GST-tagged SOMT-9, SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
quercetin + S-adenosyl-L-methionine Glycine max SOMT-9 is involved in the biosynthesis of isorhamnetin 3'-O-methylquercetin + S-adenosyl-L-homocysteine
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged SOMT-9 from Escherichia coli to homogeneity Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Glycine max
-
root
-
Glycine max
-
stem
-
Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
luteolin + S-adenosyl-L-methionine high 3'-O-methyltransferase activity Glycine max 3'-O-methyl luteolin + S-adenosyl-L-homocysteine i.e. chrysoeriol ?
additional information SOMT-9 transfers a methyl group to the 3'-OH group of substrates having ortho-hydroxyl groups and uses a flavonoids as a substrates, no activity with 3,3'-dihydroxyflavone, 5,3'-dihydroxyflavone, 6,3'-dihydroxyflavone, 3'-hydroxyflavone, and 3'-hydroxyflavanone Glycine max ?
-
?
quercetin + S-adenosyl-L-methionine SOMT-9 is involved in the biosynthesis of isorhamnetin Glycine max 3'-O-methylquercetin + S-adenosyl-L-homocysteine
-
?
quercetin + S-adenosyl-L-methionine a flavone, 3'-O-methyltransferase activity, best substrate Glycine max 3'-O-methylquercetin + S-adenosyl-L-homocysteine i.e. isorhamnetin ?
taxifolin + S-adenosyl-L-methionine 3'-O-methyltransferase activity Glycine max 3'-O-methyl taxifolin + S-adenosyl-L-homocysteine
-
?

Subunits

Subunits Comment Organism
? x * 64000, recombinant GST-tagged SOMT-9, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
3'-O-methyltransferase
-
Glycine max
SOMT-9
-
Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Glycine max

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Glycine max