Cloned (Comment) | Organism |
---|---|
SOMT-9, DNA and amino acid sequence determination, expression of GST-tagged SOMT-9 in Escherichia coli | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
x * 64000, recombinant GST-tagged SOMT-9, SDS-PAGE | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quercetin + S-adenosyl-L-methionine | Glycine max | SOMT-9 is involved in the biosynthesis of isorhamnetin | 3'-O-methylquercetin + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged SOMT-9 from Escherichia coli to homogeneity | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Glycine max | - |
root | - |
Glycine max | - |
stem | - |
Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
luteolin + S-adenosyl-L-methionine | high 3'-O-methyltransferase activity | Glycine max | 3'-O-methyl luteolin + S-adenosyl-L-homocysteine | i.e. chrysoeriol | ? | |
additional information | SOMT-9 transfers a methyl group to the 3'-OH group of substrates having ortho-hydroxyl groups and uses a flavonoids as a substrates, no activity with 3,3'-dihydroxyflavone, 5,3'-dihydroxyflavone, 6,3'-dihydroxyflavone, 3'-hydroxyflavone, and 3'-hydroxyflavanone | Glycine max | ? | - |
? | |
quercetin + S-adenosyl-L-methionine | SOMT-9 is involved in the biosynthesis of isorhamnetin | Glycine max | 3'-O-methylquercetin + S-adenosyl-L-homocysteine | - |
? | |
quercetin + S-adenosyl-L-methionine | a flavone, 3'-O-methyltransferase activity, best substrate | Glycine max | 3'-O-methylquercetin + S-adenosyl-L-homocysteine | i.e. isorhamnetin | ? | |
taxifolin + S-adenosyl-L-methionine | 3'-O-methyltransferase activity | Glycine max | 3'-O-methyl taxifolin + S-adenosyl-L-homocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, recombinant GST-tagged SOMT-9, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
3'-O-methyltransferase | - |
Glycine max |
SOMT-9 | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Glycine max |