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Literature summary for 2.1.1.45 extracted from
- The enzymatic synthesis of thymidylate. I. Early steps in the purification of thymidylate synthetase of Escherichia coli (1962), J. Biol. Chem., 237, 3794-3801.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Chloroacetamide | - |
Escherichia coli |  |
| iodoacetate | - |
Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
2'-deoxyuridylate | - |
Escherichia coli | |
| 0.0045 | - |
L-tetrahydrofolate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Enterococcus faecalis | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli B / ATCC 11303 | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.016 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate | - |
Escherichia coli | 7,8-dihydropteroylglutamate + thymidine phosphate | - |
? | |
| (-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate | - |
Escherichia coli B / ATCC 11303 | 7,8-dihydropteroylglutamate + thymidine phosphate | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | activity is higher in Tris buffer than in phosphate buffer | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.8 | phosphate buffer | Escherichia coli |
| 6.2 | 9.5 | Tris buffer | Escherichia coli |
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Release 2023.1 (January 2023)
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