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Literature summary for 2.1.1.45 extracted from
- Presence and properties of thymidylate synthase in trypanosomatids (1977), Biochim. Biophys. Acta, 481, 71-79.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | possible target for chemotherapeutic attack | Trypanosoma brucei |
| medicine | possible target for chemotherapeutic attack | Crithidia fasciculata |
| medicine | possible target for chemotherapeutic attack | Trypanosoma cruzi |
| medicine | possible target for chemotherapeutic attack | Trypanosoma congolense |
| medicine | possible target for chemotherapeutic attack | Strigomonas oncopelti |
| medicine | possible target for chemotherapeutic attack | Trypanosoma lewisi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Crithidia fasciculata |  |
| 2-mercaptoethanol | - |
Strigomonas oncopelti | |
| 2-mercaptoethanol | - |
Trypanosoma brucei | |
| 2-mercaptoethanol | - |
Trypanosoma congolense | |
| 2-mercaptoethanol | - |
Trypanosoma cruzi | |
| 2-mercaptoethanol | - |
Trypanosoma lewisi | |
| 5-fluorouracil | - |
Crithidia fasciculata | |
| 5-fluorouracil | - |
Escherichia coli | |
| 5-fluorouracil | - |
Trypanosoma cruzi | |
| aminopterin | - |
Crithidia fasciculata | |
| aminopterin | - |
Trypanosoma cruzi | |
| antrycide | - |
Crithidia fasciculata | |
| antrycide | - |
Strigomonas oncopelti | |
| antrycide | - |
Trypanosoma brucei | |
| antrycide | - |
Trypanosoma congolense | |
| antrycide | - |
Trypanosoma cruzi | |
| antrycide | - |
Trypanosoma lewisi | |
| dimidium | - |
Crithidia fasciculata | |
| dimidium | - |
Strigomonas oncopelti | |
| dimidium | - |
Trypanosoma brucei | |
| dimidium | - |
Trypanosoma congolense | |
| dimidium | - |
Trypanosoma cruzi | |
| dimidium | - |
Trypanosoma lewisi | |
| ethidium | - |
Crithidia fasciculata | |
| ethidium | - |
Strigomonas oncopelti | |
| ethidium | - |
Trypanosoma brucei | |
| ethidium | - |
Trypanosoma congolense | |
| ethidium | - |
Trypanosoma cruzi | |
| ethidium | - |
Trypanosoma lewisi | |
| Mg2+ | - |
Crithidia fasciculata | |
| Mg2+ | - |
Strigomonas oncopelti | |
| Mg2+ | - |
Trypanosoma brucei | |
| Mg2+ | - |
Trypanosoma congolense | |
| Mg2+ | - |
Trypanosoma cruzi | |
| Mg2+ | - |
Trypanosoma lewisi | |
| Pentamidine | - |
Crithidia fasciculata | |
| Pentamidine | - |
Strigomonas oncopelti | |
| Pentamidine | - |
Trypanosoma brucei | |
| Pentamidine | - |
Trypanosoma congolense | |
| Pentamidine | - |
Trypanosoma cruzi | |
| Pentamidine | - |
Trypanosoma lewisi | |
| prothidium | - |
Crithidia fasciculata | |
| prothidium | - |
Strigomonas oncopelti | |
| prothidium | - |
Trypanosoma brucei | |
| prothidium | - |
Trypanosoma congolense | |
| prothidium | - |
Trypanosoma cruzi | |
| prothidium | - |
Trypanosoma lewisi | |
| suramin | - |
Crithidia fasciculata | |
| suramin | - |
Strigomonas oncopelti | |
| suramin | - |
Trypanosoma brucei | |
| suramin | - |
Trypanosoma congolense | |
| suramin | - |
Trypanosoma cruzi | |
| suramin | - |
Trypanosoma lewisi | |
| trimethoprim | - |
Crithidia fasciculata | |
| trimethoprim | - |
Trypanosoma cruzi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 175000 | - |
gel filtration | Crithidia fasciculata |
| 200000 | - |
gel filtration | Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Rattus norvegicus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Trypanosoma brucei | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Crithidia fasciculata | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Trypanosoma cruzi | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Trypanosoma congolense | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Strigomonas oncopelti | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Trypanosoma lewisi | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Trypanosoma brucei gambiense | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Trypanosoma rhodesiense | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
host Anopheles strain ATCC 11,745 | - |
| Escherichia coli | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Strigomonas oncopelti | - |
- |
- |
| Trypanosoma brucei | - |
- |
- |
| Trypanosoma brucei gambiense | - |
- |
- |
| Trypanosoma congolense | - |
causes nagana, the equivalent disease of cattle | - |
| Trypanosoma cruzi | - |
sonya strain, maintained in ASH/XP mice | - |
| Trypanosoma cruzi | - |
causes Chagas' disease in man | - |
| Trypanosoma lewisi | - |
natural pathogen of rats | - |
| Trypanosoma rhodesiense | - |
causes human sleeping sickness | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | intracellular parasites, host blood | Trypanosoma brucei | - |
| blood | intracellular parasites, host blood | Crithidia fasciculata | - |
| blood | intracellular parasites, host blood | Trypanosoma cruzi | - |
| blood | intracellular parasites, host blood | Trypanosoma congolense | - |
| blood | intracellular parasites, host blood | Strigomonas oncopelti | - |
| blood | intracellular parasites, host blood | Trypanosoma lewisi | - |
| blood | obtained from infected rat blood | Trypanosoma brucei | - |
| blood | obtained from infected rat blood | Trypanosoma congolense | - |
| blood | obtained from infected rat blood | Trypanosoma lewisi | - |
| fetus | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00012 | - |
- |
Trypanosoma brucei |
| 0.00014 | - |
- |
Trypanosoma congolense |
| 0.00035 | - |
intracellular form | Trypanosoma cruzi |
| 0.00038 | - |
- |
Trypanosoma lewisi |
| 0.00054 | - |
- |
Strigomonas oncopelti |
| 0.00119 | - |
blood form | Trypanosoma cruzi |
| 0.00156 | - |
culture form | Trypanosoma cruzi |
| 0.00218 | - |
- |
Crithidia fasciculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma brucei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma brucei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma cruzi | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma cruzi | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma congolense | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma congolense | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Strigomonas oncopelti | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Strigomonas oncopelti | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma lewisi | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma lewisi | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma brucei gambiense | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma brucei gambiense | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma rhodesiense | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma rhodesiense | dihydrofolate + dTMP | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Crithidia fasciculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 6.7 | - |
Trypanosoma lewisi |
| 6.5 | 6.7 | - |
Trypanosoma brucei gambiense |
| 7.8 | - |
- |
Crithidia fasciculata |
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