KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
2'-deoxyuridylate | - |
Escherichia coli | |
0.0104 | - |
2'-deoxyuridylate | - |
Escherichia coli | |
0.014 | - |
5,10-methylene-5,6,7,8-tetrahydrofolate | - |
Escherichia coli | |
0.11 | - |
2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli | |
0.113 | - |
2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Streptococcus pneumoniae | |
Mg2+ | - |
Lacticaseibacillus casei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000 | Escherichia coli |
32500 | - |
2 * 32500, SDS-PAGE | Escherichia coli |
63000 | 64000 | gel filtration | Escherichia coli |
64000 | - |
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Streptococcus pneumoniae | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia phage T2 | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli B / ATCC 11303 | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli EM 20031 K12 | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Escherichia coli EM 20031 K12 | - |
- |
- |
Escherichia phage T2 | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.47 | - |
- |
Escherichia coli |
1.52 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, enzyme can be stored frozen for several months without significant loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli | 7,8-dihydrofolate + ? | - |
? | |
5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli B / ATCC 11303 | 7,8-dihydrofolate + ? | - |
? | |
5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate | - |
Escherichia coli EM 20031 K12 | 7,8-dihydrofolate + ? | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Streptococcus pneumoniae | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia phage T2 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli B / ATCC 11303 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli EM 20031 K12 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli EM 20031 K12 | dihydrofolate + dTMP | - |
? | |
additional information | - |
Streptococcus pneumoniae | ? | - |
? | |
additional information | - |
Lacticaseibacillus casei | ? | - |
? | |
additional information | nucleotides with large substituents on the 2' position are no substrates | Escherichia coli | ? | - |
? | |
additional information | enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates | Escherichia coli | ? | - |
? | |
additional information | nucleotides with large substituents on the 2' position are no substrates | Escherichia coli B / ATCC 11303 | ? | - |
? | |
additional information | enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates | Escherichia coli B / ATCC 11303 | ? | - |
? | |
additional information | nucleotides with large substituents on the 2' position are no substrates | Escherichia coli EM 20031 K12 | ? | - |
? | |
additional information | enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates | Escherichia coli EM 20031 K12 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Streptococcus pneumoniae |
dimer | - |
Lacticaseibacillus casei |
dimer | - |
Escherichia phage T2 |
dimer | 2 * 32500, SDS-PAGE | Escherichia coli |
dimer | 2 * 32000 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Escherichia coli |