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Literature summary for 2.1.1.45 extracted from
- Thymidylate synthetase from Saccharomyces cerevisiae. Purification and enzymic properties (1981), J. Biol. Chem., 256, 12456-12462.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-bromo-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae |  |
| 5-fluoro-2'-deoxyuridylic acid | - |
Enterococcus faecalis | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
| aminopterin | - |
Saccharomyces cerevisiae | |
| Cibacron blue | - |
Saccharomyces cerevisiae | |
| dTMP | - |
Saccharomyces cerevisiae | |
| methotrexate | - |
Homo sapiens | |
| methotrexate | - |
Saccharomyces cerevisiae | |
| thymidine phosphate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
2'-deoxyuridylate | - |
Saccharomyces cerevisiae | |
| 0.07 | - |
(-)-5,10-methylene-tetrahydropteroylglutamate | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Saccharomyces cerevisiae |
| 65000 | - |
gel filtration, velocity sedimentation electrophoresis | Saccharomyces cerevisiae |
| 68000 | - |
gel filtration | Saccharomyces cerevisiae |
| 69000 | - |
Ehrlich ascites carcinoma cells | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Gallus gallus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Saccharomyces cerevisiae | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Enterococcus faecalis | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
- |
- |
| Gallus gallus | - |
chicken | - |
| Homo sapiens | - |
- |
- |
| Lacticaseibacillus casei | - |
- |
- |
| Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
| Saccharomyces cerevisiae | - |
bakers' yeast | - |
| Saccharomyces cerevisiae | - |
haploid strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blast cell | - |
Homo sapiens | - |
| embryo | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.2 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Enterococcus faecalis | dihydrofolate + dTMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Mus musculus |
| dimer | - |
Homo sapiens |
| dimer | - |
Lacticaseibacillus casei |
| dimer | 2 * 30000, SDS-PAGE | Saccharomyces cerevisiae |
| monomer | 1 * 69000, Ehrlich ascites carcinoma cells | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.2 | - |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8.5 | half-maximal activity at either pH 5.8 or pH 8.5 | Saccharomyces cerevisiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000004 | - |
Cibacron blue | - |
Saccharomyces cerevisiae | |
| 0.000005 | - |
5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
| 0.01 | - |
5-bromo-2'-deoxyuridine 5'-monophosphate | - |
Saccharomyces cerevisiae | |
| 0.015 | - |
aminopterin | - |
Saccharomyces cerevisiae | |
| 0.02 | - |
methotrexate | - |
Saccharomyces cerevisiae | |
| 0.02 | - |
aminopterin | - |
Saccharomyces cerevisiae | |
| 0.08 | - |
thymidine phosphate | - |
Saccharomyces cerevisiae | |
| 0.1 | - |
dTMP | - |
Saccharomyces cerevisiae | |
| 0.12 | - |
thymidine phosphate | - |
Saccharomyces cerevisiae | |
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