Application | Comment | Organism |
---|---|---|
medicine | - |
Mus musculus |
medicine | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
expression vector pETSM, cloned and expressed in Escherichia coli BL21(DE3)pLysS | Mus musculus |
General Stability | Organism |
---|---|
glycerol and Triton X-100 stabilize the enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Lacticaseibacillus casei | - |
- |
- |
Mus musculus | - |
L1210 cells | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
affinity chromatography | Mus musculus |
1.33 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, activity remains unchanged after 1 month | Mus musculus |
-20°C, in presence of 0.1% Triton X-100 and 10% glycerol, no loss of activity when stored in lysis buffer for several weeks | Mus musculus |
4°C, in presence of 0.1% Triton X-100 and 10% glycerol, no loss of activity when stored in lysis buffer for at least 2 weeks | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Mus musculus |