Application | Comment | Organism |
---|---|---|
medicine | - |
Mus musculus |
medicine | - |
Homo sapiens |
medicine | - |
Tequatrovirus T4 |
medicine | - |
Pneumocystis carinii |
medicine | drug development against cryptococcosis, a pulmonary subclinical infection, in immunocompromised individuals having diseases such as leukemia, lymphoma or AIDS the infection seminates to the central nervous system, the fourth most common opportunistic infection complicating AIDS | Cryptococcus neoformans |
Cloned (Comment) | Organism |
---|---|
- |
Pneumocystis carinii |
cDNA of TS gene subcloned into T7 polymerase vector pET15-b, production of active TS by complementation of Escherichia coli | Cryptococcus neoformans |
General Stability | Organism |
---|---|
stable upon quick-freezing in liquid nitrogen in buffer containing 150 mM KCl | Cryptococcus neoformans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroseleno-2'-deoxyuridylic acid | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
2'-deoxyuridylate | - |
Cryptococcus neoformans | |
0.0382 | - |
5,10-methylenetetrahydrofolate | - |
Cryptococcus neoformans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Cryptococcus neoformans |
35840 | - |
cDNA sequence | Cryptococcus neoformans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Tequatrovirus T4 | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Candida albicans | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Pneumocystis carinii | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Cryptococcus neoformans | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Cryptococcus neoformans B-3501A | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Cryptococcus neoformans | P0CS13 | encapsulated yeast-like fungus, GenBank accession No. | - |
Cryptococcus neoformans B-3501A | P0CS13 | encapsulated yeast-like fungus, GenBank accession No. | - |
Homo sapiens | - |
human | - |
Lacticaseibacillus casei | - |
- |
- |
Mus musculus | - |
- |
- |
Pneumocystis carinii | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed in E. coli | Cryptococcus neoformans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
- |
Cryptococcus neoformans |
Storage Stability | Organism |
---|---|
-20°C, 40% glycerol, stable over 2 months, 50% loss of activity by 24 h storage without glycerol | Cryptococcus neoformans |
-80°C, 40% glycerol, stable over 2 months | Cryptococcus neoformans |
-80°C, stable upon storage in Tris-HCl pH 7.4 and thiol | Cryptococcus neoformans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Candida albicans | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Candida albicans | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Pneumocystis carinii | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Cryptococcus neoformans | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Pneumocystis carinii | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Cryptococcus neoformans | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Cryptococcus neoformans B-3501A | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Cryptococcus neoformans B-3501A | dihydrofolate + dTMP | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mus musculus |
7 | - |
- |
Cryptococcus neoformans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.4 | 8.6 | 50% activity at pH 6.4 and 8.6 | Cryptococcus neoformans |