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Literature summary for 2.1.1.45 extracted from

  • Liang, P.H.; Anderson, K.S.
    Substrate channeling and domain-domain interactions in bifunctional thymidylate synthase-dihydrofolate reductase (1998), Biochemistry, 37, 12195-12205.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
clone harbors the pO2CLSA-4 plasmid expressed in an Escherichia coli Rue 10 expression vector Leishmania major

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
5-fluoro-2'-deoxyuridylic acid
-
Escherichia coli
5-fluoro-2'-deoxyuridylic acid
-
Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Leishmania major
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Toxoplasma gondii
-
dihydrofolate + dTMP
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Leishmania major
-
formerly called Leishmania tropica
-
Toxoplasma gondii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6S)-tetrahydropteroylglutamate + 2-deoxyuridylate
-
Leishmania major 7,8-dihydropteroylglutamate + thymidine phosphate
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Leishmania major dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Leishmania major dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Toxoplasma gondii dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Toxoplasma gondii dihydrofolate + dTMP
-
r
additional information
-
Escherichia coli ?
-
?
additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Leishmania major ?
-
?
additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Toxoplasma gondii ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.67
-
2'-deoxyuridine
-
Leishmania major