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Literature summary for 2.1.1.45 extracted from

  • Mahdavian, E.; Spencer, H.T.; Dunlap, R.B.
    Kinetic studies on drug-resistant variants of Escherichia coli thymidylate sythase: Functional effects of amino acid sunstitutions at residue 4 (1999), Arch. Biochem. Biophys., 368, 257-264.
    View publication on PubMed
Search for more literature for 2.1.1.45

Application

Application Comment Organism
medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 2'-deoxyuridylate wild-type Escherichia coli
0.0012
-
 2'-deoxyuridylate mutant Y4F Escherichia coli
0.0014
-
 2'-deoxyuridylate mutant Y4I, Y4V Escherichia coli
0.003
-
 2'-deoxyuridylate mutant Y4H Escherichia coli
0.008
-
 methylenetetrahydrofolate mutant Y4F Escherichia coli
0.01
-
 methylenetetrahydrofolate wild-type Escherichia coli
0.013
-
 methylenetetrahydrofolate mutant Y4H Escherichia coli
0.015
-
 methylenetetrahydrofolate mutant Y4V Escherichia coli
0.018
-
 methylenetetrahydrofolate mutant Y4I Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Escherichia coli key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
 human
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Escherichia coli 7,8-dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Escherichia coli dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
 ?

Cofactor

Cofactor Comment Organism Structure
N5,N10-methylenetetrahydrofolate
-
 Homo sapiens