Application | Comment | Organism |
---|---|---|
medicine | thymidylate synthase is an important target of several chemotherapeutic agents including 5-fluorouracil and raltitrexed | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a cell line expressing the Ugi protein inhibitor of UNG family of uracil DNA glycosylases, which reduces cellular uracil DNA glycosylase activity by at least 45-fold. Genomic uracil levels are increased over 4fold following thymidylate synthase inhibition in the Ugi-expressing cells, but do not detectably increase in UNG proficient cells. No difference in toxicity between the UNG proficient and UNG-inhibited cells to folate or nucleotide-based inhibitors of thymidylate synthase. UNG proficient and UNG-inhibited cells arrest in early S-phase and resume replication progression during recovery from raltitrexed treatment almost identically | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluorouracil | 5-FU | Homo sapiens | |
capecitabine | prodrug of 5-fluorouracil | Homo sapiens | |
raltitrexed | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5,N10-methylenetetrahydrofolate + dUMP | Homo sapiens | during thymidylate synthase inhibition, TMP levels decrease with a subsequent increase in dUTP | 7,8-dihydrofolate + TMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | additionally transfected with pLGC-hUgi and pLGCX plasmids | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5,N10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | 7,8-dihydrofolate + TMP | - |
? | |
N5,N10-methylenetetrahydrofolate + dUMP | during thymidylate synthase inhibition, TMP levels decrease with a subsequent increase in dUTP | Homo sapiens | 7,8-dihydrofolate + TMP | - |
? |